The Role Played by Adenosine in Modulating Reflex Sympathetic and Pressor Responses Evoked by Stimulation of TRPV1 in Muscle Afferents.
The Role Played by Adenosine in Modulating Reflex Sympathetic and Pressor Responses Evoked by Stimulation of TRPV1 in Muscle Afferents.
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DOI：
10.1159/000452523
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Xing J;Li J
大鼠后肢肌肉传入神经的代谢物敏感的瞬时受体香草素1型受体(TRPV1受体)的激活通过反射机制增加肾交感神经活动(RSNA)和血压(BP)。本研究的目的是研究腺苷在调节反射性RSNA和BP对TRPV1刺激的反应中的作用。记录大鼠的RSNA和BP反应。采用免疫荧光和膜片钳技术研究腺苷作用的受体机制。股动脉注射100µM腺苷对辣椒素诱发的反射性RSNA和BP反应有抑制作用。同样，动脉注射腺苷类似物CGS21680(A2A型受体激动剂，10µM和100µM)也可减弱反射反应。此外，A2A和TRPV1在背根神经节神经元中共存。预先应用腺苷或CGS21680可抑制辣椒素诱导的肌肉感觉神经元电流的幅度。腺苷参与肌肉传入TRPV1的反射、交感和升压反应。在疾病的情况下，后肢肌肉中的腺苷水平增加，可能会损害TRPV1的反应。
Activation of metabolite-sensitive transient receptor potential vanilloid type 1 (TRPV1) receptors (capsaicin receptors) in afferent nerves of the hindlimb muscles of rats increases renal sympathetic nerve activity (RSNA) and blood pressure (BP) via a reflex mechanism. The purpose of this study was to examine the role of adenosine in modulating the reflex RSNA and BP responses to stimulation of TRPV1. RSNA and BP responses were recorded in rats. Immunofluorescence and patch-clamp methods were employed to examine the receptor mechanisms responsible for the effects of adenosine. Adenosine, in the concentration of 100 µM, injected into the femoral artery had an inhibitory effect on the reflex RSNA and BP responses induced by capsaicin. Likewise, arterial injection of adenosine analogue CGS21680 (A2A subtype receptor agonist, 10 µM and 100 µM) also attenuated the reflex responses. In addition, co-existence of A2A and TRPV1 was observed in the dorsal root ganglion neurons. The prior application of adenosine or CGS21680 inhibited the magnitude of capsaicin-induced currents in muscle sensory neurons. Adenosine contributes to muscle afferent TRPV1-engaged reflex sympathetic and pressor responses. It is likely that TRPV1 response is impaired as the levels of adenosine are increased in the hindlimb muscles under diseased conditions.
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影响因子：
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