Distinct human stem cell populations in small and large intestine.
Distinct human stem cell populations in small and large intestine.
复制标题
DOI：
10.1371/journal.pone.0118792
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Lagasse E
中科院分区：
文献类型：
--
作者：
Cramer JM;Thompson T;Geskin A;LaFramboise W;Lagasse E
肠由含有快速增殖的细胞的上皮层组成，这些细胞成熟为两个区域，即小肠和大肠。尽管之前的研究已经确定干细胞是肠上皮细胞的起源细胞，但还没有研究直接比较来自这些解剖学上不同区域的干细胞。在这里，我们使用Wnt激动剂R-Respondin 2检测从人胚胎小肠和大肠分离的原代上皮细胞在体外扩增后的内在差异。我们使用流式细胞术、荧光激活细胞分类、基因表达分析和三维体外分化实验来表征它们的干细胞特性。通过基因表达分析，我们发现了在小肠和大肠中分离集落形成细胞亚群的干细胞标记，并揭示了在分化、增殖和疾病途径方面的重要差异。来自小肠和大肠培养的单个细胞形成了有机类化合物，反映了体内发现的不同的细胞层次结构，并对相同的外源线索做出了不同的反应。我们的研究结果表明，小肠和大肠上皮干细胞之间存在许多差异，提示它们可能与肠道疾病有关。
The intestine is composed of an epithelial layer containing rapidly proliferating cells that mature into two regions, the small and the large intestine. Although previous studies have identified stem cells as the cell-of-origin for intestinal epithelial cells, no studies have directly compared stem cells derived from these anatomically distinct regions. Here, we examine intrinsic differences between primary epithelial cells isolated from human fetal small and large intestine, after in vitro expansion, using the Wnt agonist R-spondin 2. We utilized flow cytometry, fluorescence-activated cell sorting, gene expression analysis and a three-dimensional in vitro differentiation assay to characterize their stem cell properties. We identified stem cell markers that separate subpopulations of colony-forming cells in the small and large intestine and revealed important differences in differentiation, proliferation and disease pathways using gene expression analysis. Single cells from small and large intestine cultures formed organoids that reflect the distinct cellular hierarchy found in vivo and respond differently to identical exogenous cues. Our characterization identified numerous differences between small and large intestine epithelial stem cells suggesting possible connections to intestinal disease.
登录
查看更多内容
影响因子：
11.2
作者：
Odoux, Christine;Fohrer, Helene;Hoppo, Toshitaka;Guzik, Lynda;Stolz, Donna Beer;Lewis, Dale W.;Gollin, Susanne M.;Gamblin, T. Clark;Geller, David A.;Lagasse, Eric
通讯作者：
Lagasse, Eric
影响因子：
--
作者：
Kazanjian, Avedis;Shroyer, Noah F
通讯作者：
Shroyer, Noah F
DOI：
10.1083/jcb.98.3.1026
发表时间：
1984-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ehmann UK;Peterson WD Jr;Misfeldt DS
通讯作者：
Misfeldt DS
影响因子：
64.8
作者：
O'Brien, Catherine A.;Pollett, Aaron;Dick, John E.
通讯作者：
Dick, John E.
影响因子：
9.8
作者：
Bossuyt W;Kazanjian A;De Geest N;Van Kelst S;De Hertogh G;Geboes K;Boivin GP;Luciani J;Fuks F;Chuah M;VandenDriessche T;Marynen P;Cools J;Shroyer NF;Hassan BA
通讯作者：
Hassan BA