Tempol differently affects cellular redox changes and antioxidant enzymes in various lung-related cells.
Tempol differently affects cellular redox changes and antioxidant enzymes in various lung-related cells.
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DOI：
10.1038/s41598-021-94340-z
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发表时间：
2021-07-21
影响因子：
4.6
通讯作者：
Park WH
中科院分区：
文献类型：
--
作者：
Park WH
Tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl) is a potential redox agent in cells. The present study investigated changes in cellular reactive oxygen species (ROS) and glutathione (GSH) levels and in antioxidant enzymes, in Tempol-treated Calu-6 and A549 lung cancer cells, normal lung WI-38 VA-13 cells, and primary pulmonary fibroblasts. Results demonstrated that Tempol (0.5–4 mM) either increased or decreased general ROS levels in lung cancer and normal cells at 48 h and specifically increased O2•− levels in these cells. In addition, Tempol differentially altered the expression and activity of antioxidant enzymes such as superoxide dismutase, catalase, and thioredoxin reductase1 (TrxR1) in A549, Calu-6, and WI-38 VA-13 cells. In particular, Tempol treatment increased TrxR1 protein levels in these cells. Tempol at 1 mM inhibited the growth of lung cancer and normal cells by about 50% at 48 h but also significantly induced cell death, as evidenced by annexin V-positive cells. Furthermore, down-regulation of TrxR1 by siRNA had some effect on ROS levels as well as cell growth inhibition and death in Tempol-treated or -untreated lung cells. In addition, some doses of Tempol significantly increased the numbers of GSH-depleted cells in both cancer cells and normal cells at 48 h. In conclusion, Tempol differentially increased or decreased levels of ROS and various antioxidant enzymes in lung cancer and normal cells, and induced growth inhibition and death in all lung cells along with an increase in O2•− levels and GSH depletion.
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影响因子：
4.2
作者：
Park, Woo Hyun
通讯作者：
Park, Woo Hyun
影响因子：
4.5
作者：
Park, Woo Hyun;Han, Bo Ran;Kim, Sung Zoo
通讯作者：
Kim, Sung Zoo
影响因子：
5.3
作者：
Han, Yong Hwan;Kim, Sung Zoo;Park, Woo Hyun
通讯作者：
Park, Woo Hyun
DOI：
10.1007/978-1-4939-8861-7_1
发表时间：
2019-01-01
期刊：
BCL-2 FAMILY PROTEINS
影响因子：
--
作者：
Huska, Jason D.;Lamb, Heather M.;Hardwick, J. Marie
通讯作者：
Hardwick, J. Marie
影响因子：
4.2
作者：
Lauterburg, Bernhard H
通讯作者：
Lauterburg, Bernhard H