A self-regulating feed-forward circuit controlling C. elegans egg-laying behavior.
A self-regulating feed-forward circuit controlling C. elegans egg-laying behavior.
复制标题
一个自我调节的馈电回路，控制了秀丽隐杆线虫卵形的行为。
DOI：
10.1016/j.cub.2008.08.047
复制
发表时间：
2008-10-14
期刊：
影响因子：
9.2
通讯作者：
Schafer, William R.
中科院分区：
文献类型：
--
作者：
Zhang, Mi;Chung, Samuel H.;Fang-Yen, Chris;Craig, Caroline;Kerr, Rex A.;Suzuki, Hiroshi;Samuel, Aravinthan D. T.;Mazur, Eric;Schafer, William R.
秀丽隐杆线虫的产卵已经在遗传和行为水平上得到了很好的研究。然而，产卵行为的神经基础仍然不清楚；特别是特定神经元的作用和突触连接在产卵回路中的功能性质仍然不清楚。我们使用了活体神经成像和激光手术来解决这些问题，在完好无损、行为正常的动物身上。我们发现，HSN神经元通过直接兴奋外阴肌和Vc运动神经元，在驱动产卵行为中发挥核心作用。VC神经元在产卵环路中起双重作用，兴奋外阴肌，同时反馈抑制HSN。有趣的是，在没有突触输入的情况下，HSN是活跃的，这表明产卵可能是通过调节自主HSN的活动来控制的。事实上，身体接触似乎部分通过干扰HSN的钙振荡来抑制产卵。产卵电机电路由一个简单的前馈励磁和反馈抑制三分量系统组成。这种微电路基序在线虫神经系统和哺乳动物皮质中很常见；因此，了解它在线虫中的功能特性可能有助于深入了解它在更复杂的大脑中的计算作用。
Egg-laying in Caenorhabditis elegans has been well studied at the genetic and behavioral levels. However, the neural basis of egg-laying behavior is still not well understood; in particular, the roles of specific neurons and the functional nature of the synaptic connections in the egg-laying circuit remain uncharacterized. We have used in vivo neuroimaging and laser surgery to address these questions in intact, behaving animals. We have found that the HSN neurons play a central role in driving egg-laying behavior through direct excitation of the vulval muscles and VC motorneurons. The VC neurons play a dual role in the egg-laying circuit, exciting the vulval muscles while feedback-inhibiting the HSNs. Interestingly, the HSNs are active in the absence of synaptic input, suggesting that egg-laying may be controlled through modulation of autonomous HSN activity. Indeed, body touch appears to inhibit egg-laying in part by interfering with HSN calcium oscillations. The egg-laying motor circuit comprises a simple three-component system combining feed-forward excitation and feedback inhibition. This microcircuit motif is common in the C. elegans nervous system as well as in the mammalian cortex; thus, understanding its functional properties in C. elegans may provide insight into its computational role in more complex brains.
登录
查看更多内容
影响因子：
56.9
作者：
Milo, R;Shen-Orr, S;Alon, U
通讯作者：
Alon, U
影响因子：
5.3
作者：
Moresco, JJ;Koelle, MR
通讯作者：
Koelle, MR
影响因子：
1.9
作者：
HELLGREN, J;GRILLNER, S;LANSNER, A
通讯作者：
LANSNER, A
影响因子：
3.1
作者：
GomezSaladin, E;Luebke, AE;Dickerson, IM
通讯作者：
Dickerson, IM
影响因子：
4.7
作者：
Husson, Steven J.;Janssen, Tom;Schoofs, Liliane
通讯作者：
Schoofs, Liliane