Isolation of a monoclonal antibody from a phage display library binding the rhesus macaque MHC class I allomorph Mamu-A1*001.
Isolation of a monoclonal antibody from a phage display library binding the rhesus macaque MHC class I allomorph Mamu-A1*001.
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DOI：
10.1371/journal.pone.0179039
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Reynolds MR
中科院分区：
文献类型：
--
作者：
Holman N;Weinfurter JT;Harsla TR;Wiseman RW;Belli AJ;Michaels AJ;Reimann KA;DeMars RI;Reynolds MR
结合人类白细胞抗原（HLA）的单克隆抗体是用于HLA分型、骨髓移植后追踪供体-受体嵌合体以及表征细胞表面上的特异性主要组织相容性复合体（MHC）的有用工具。遗憾的是，恒河猴是器官移植和传染病研究中常用的动物模型，目前还没有等效的试剂。为了解决这一缺陷，我们分离出一种抗体，它可以识别常见的印度恒河猴MHC I类分子Mamu-A1*001。我们通过用来自雄性Mamu-A1*001阳性供体的外周血单核细胞（PBMC）同种免疫雌性Mamu-A1 * 001阴性恒河猴来诱导Mamu-A1 * 001结合抗体。用来自同种免疫猕猴的PBMC构建Fab噬菌体展示库，并淘选以分离与Mamu-A1*001结合但不与其他常见恒河猴MHC I类分子结合的抗体。分离的抗体区分PBMC与Mamu-A1*001阳性和阴性猕猴。此外，Mamu-A1*001特异性抗体结合食蟹猴MHC I类直系同源物Mafa-A1*001：01，但不结合变体Mafa-A1*001：02/03，表明高度的结合特异性。Mamu-A1*001特异性抗体将用于鉴定Mamu-A1*001阳性恒河猴，用于检测Mamu-A1 *001阴性细胞群体中的Mamu-A1*001阳性细胞，以及用于检查改变细胞表面上Mamu-A1*001表达的疾病过程。此外，我们描述的同种免疫过程将用于分离额外的MHC同种异型特异性单克隆抗体或针对其他多态性宿主蛋白的抗体，这些抗体难以用传统技术分离。
Monoclonal antibodies that bind to human leukocyte antigen (HLA) are useful tools for HLA-typing, tracking donor-recipient chimerisms after bone marrow transplants, and characterizing specific major histocompatibility complexes (MHC) on cell surfaces. Unfortunately, equivalent reagents are not available for rhesus macaques, which are commonly used animal as models in organ transplant and infectious disease research. To address this deficiency, we isolated an antibody that recognizes the common Indian rhesus macaque MHC class I molecule, Mamu-A1*001. We induced Mamu-A1*001-binding antibodies by alloimmunizing a female Mamu-A1*001-negative rhesus macaque with peripheral blood mononuclear cells (PBMC) from a male Mamu-A1*001-positive donor. A Fab phage display library was constructed with PBMC from the alloimmunized macaque and panned to isolate an antibody that binds to Mamu-A1*001 but not to other common rhesus macaque MHC class I molecules. The isolated antibody distinguishes PBMC from Mamu-A1*001-positive and -negative macaques. Additionally, the Mamu-A1*001-specific antibody binds the cynomolgus macaque MHC class I ortholog Mafa-A1*001:01 but not variants Mafa-A1*001:02/03, indicating a high degree of binding specificity. The Mamu-A1*001-specific antibody will be useful for identifying Mamu-A1*001-positive rhesus macaques, for detecting Mamu-A1*001-positive cells in populations of Mamu-A1*001-negative cells, and for examining disease processes that alter expression of Mamu-A1*001 on cell surfaces. Moreover, the alloimmunization process we describe will be useful for isolating additional MHC allomorph-specific monoclonal antibodies or antibodies against other polymorphic host proteins which are difficult to isolate with traditional technologies.
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影响因子：
3.2
作者：
de Groot, Natasja G.;Otting, Nel;Robinson, James;Blancher, Antoine;Lafont, Bernard A. P.;Marsh, Steven G. E.;O'Connor, David H.;Shiina, Takashi;Walter, Lutz;Watkins, David I.;Bontrop, Ronald E.
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Bontrop, Ronald E.
影响因子：
2.7
作者：
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通讯作者：
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影响因子：
8.8
作者：
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通讯作者：
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影响因子：
3.2
作者：
Dudley, Dawn M.;Karl, Julie A.;Creager, Hannah M.;Bohn, Patrick S.;Wiseman, Roger W.;O'Connor, David H.
通讯作者：
O'Connor, David H.
影响因子：
3.2
作者：
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通讯作者：
O'Connor, David H.