The tendon-to-bone transition of the rotator cuff: a preliminary Raman spectroscopic study documenting the gradual mineralization across the insertion in rat tissue samples.
The tendon-to-bone transition of the rotator cuff: a preliminary Raman spectroscopic study documenting the gradual mineralization across the insertion in rat tissue samples.
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DOI：
10.1366/000370208786822179
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发表时间：
2008-12
影响因子：
3.5
通讯作者：
Thomopoulos S
中科院分区：
文献类型：
--
作者：
Wopenka B;Kent A;Pasteris JD;Yoon Y;Thomopoulos S
我们应用拉曼光谱监测了五只大鼠肩部肌腱-骨植入部位的矿物质分布和矿化程度。我们在每个组织样本的12 0Δm宽的过渡区的单个1μm点上获得了10 0~4 0 0μcm-1的拉曼光谱，并识别了在纯腱和纯骨中以及在过渡区中检测到的所有峰。磷灰石的960Δcm~(-1)P-O拉伸强度(归一化为2940Δcm~(-1)C-H拉伸或1003Δcm~(-1)C-C拉伸的胶原)被用作矿物丰度的指标。我们将观察到的组织薄片的组织形态与观察到的有机成分(主要是胶原)和无机成分(矿物磷灰石的碳化形式)的拉曼峰联系起来，并讨论了与峰的去卷积和重叠拉曼峰的量化有关的光谱问题。我们发现，在从肌腱到骨骼的120μm的距离上，植入部位的矿物质与胶原的比率呈线性增加(5个样本的R2=0.8%)，而不是像以前从组织学观察中推断的那样突然增加。此外，960Δcm-1谱带的变窄表明，磷灰石中的结晶有序性随着矿化程度的增加而增加。这一矿物质级配的发现具有重要的临床意义，并可能解释为什么肩袖未受损伤的肌腱与骨的连接可以维持非常高的应力集中而不会失败。我们的发现也与最近开发的力学模型和计算一致，这些模型和计算是为了更好地理解这种异常强烈的界面的材料性质。
We applied Raman spectroscopy to monitor the distribution of minerals and the degree of mineralization across the tendon–bone insertion site in the shoulders of five rats. We acquired Raman spectra from 100 to 4000 Δcm-1 on individual 1 μm points across the 120 μm wide transition zone of each tissue sample and identified all the peaks detected in pure tendon and in pure bone, as well as in the transition zone. The intensity of the 960 Δcm-1 P–O stretch for apatite (normalized to either the 2940 Δcm-1 C–H stretch or the 1003 Δcm-1 C–C stretch for collagen) was used as an indicator of the abundance of mineral. We relate the observed histological morphology in the tissue thin section with the observed Raman peaks for both the organic component (mostly collagen) and the inorganic component (a carbonated form of the mineral apatite) and discuss spectroscopic issues related to peak deconvolution and quantification of overlapping Raman peaks. We show that the mineral-to-collagen ratio at the insertion site increases linearly (R2 = 0.8 for five samples) over the distance of 120 μm from tendon to bone, rather than abruptly, as previously inferred from histological observations. In addition, narrowing of the 960 Δcm-1 band across the traverse indicates that the crystalline ordering within the apatite increases concomitantly with the degree of mineralization. This finding of mineral gradation has important clinical implications and may explain why the uninjured tendon-to-bone connection of the rotator cuff can sustain very high stress concentrations without failure. Our finding is also consistent with recent mechanical models and calculations developed to better understand the materials properties of this unusually strong interface.
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影响因子：
3.4
作者：
Golcuk, Kurtulus;Mandair, GuiJit S.;Morris, Michael D.
通讯作者：
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影响因子：
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作者：
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作者：
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通讯作者：
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作者：
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作者：
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通讯作者：
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