Origin, maturation, and astroglial transformation of secondary radial glial cells in the developing dentate gyrus.
Origin, maturation, and astroglial transformation of secondary radial glial cells in the developing dentate gyrus.
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DOI：
10.1002/glia.21029
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发表时间：
2010-10
期刊：
影响因子：
6.2
通讯作者：
Bock, Hans H.
中科院分区：
文献类型：
--
作者：
Brunne, Bianka;Zhao, Shanting;Derouiche, Amin;Herz, Joachim;May, Petra;Frotscher, Michael;Bock, Hans H.
齿状回是大脑的一个区域，在那里神经元在人的一生中不断诞生。在成人中，其放射状胶质细胞在神经发生中的作用近年来引起了人们的广泛关注，然而，在这种大脑结构的个体发育过程中，胶质细胞的产生和分化以及它们与放射状胶质细胞的关系却知之甚少。在这里，我们结合免疫组织化学表型，使用针对胶质标记蛋白的抗体和BrdU出生测定来表征齿状回中继发性放射状胶质支架的发育及其向星形胶质细胞分化的潜力。我们证明，BLBP、GLAST和GFAP的表达是出生后早期齿状回中未成熟分化细胞局限于星形细胞命运的特征。基于我们的研究，我们提出了一个未成熟星形胶质细胞通过颗粒细胞层径向迁移到齿状回分子层的最终位置的模型。hgap - egfp转基因小鼠的急性海马切片延时成像为这种在发育中的齿状回中分化星形胶质细胞的迁移模式提供了直接证据。
The dentate gyrus is a brain region where neurons are continuously born throughout life. In the adult, the role of its radial glia in neurogenesis has attracted much attention over the past years, however, little is known about the generation and differentiation of glial cells and their relationship to radial glia during the ontogenetic development of this brain structure. Here, we combine immunohistochemical phenotyping using antibodies against glial marker proteins with BrdU birthdating to characterize the development of the secondary radial glial scaffold in the dentate gyrus and its potential to differentiate into astrocytes. We demonstrate that the expression of BLBP, GLAST and GFAP characterizes immature differentiating cells confined to an astrocytic fate in the early postnatal dentate gyrus. Based on our studies we propose a model where immature astrocytes migrate radially through the granule cell layer to adopt their final positions in the molecular layer of the dentate gyrus. Time-lapse imaging of acute hippocampal slices from hGFAP-eGFP transgenic mice provide direct evidence for such a migration mode of differentiating astroglial cells in the developing dentate gyrus.
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