Blochmannia endosymbionts improve colony growth and immune defence in the ant Camponotus fellah.
Blochmannia endosymbionts improve colony growth and immune defence in the ant Camponotus fellah.
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DOI：
10.1186/1471-2180-9-29
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发表时间：
2009-02-06
期刊：
影响因子：
4.2
通讯作者：
Lenoir A
中科院分区：
文献类型：
--
作者：
de Souza DJ;Bézier A;Depoix D;Drezen JM;Lenoir A
微生物是一种庞大而多样的生命形式。其中许多与大型多细胞生物共生，与宿主发展共生关系，有些甚至进化形成专性内共生。迄今为止研究的所有木蚁（弓背蚁属）都含有布洛曼尼亚属的主要内共生细菌。这些细菌在蚂蚁营养中的作用已被证明，但这些蚂蚁的杂食性饮食使我们推测这些细菌可能为其宿主提供额外的优势。在这项研究中，我们建立了布洛曼尼亚、新菌落的生长和宿主免疫反应之间的联系。我们通过在蜂蜜溶液中混合一定剂量的抗生素（利福平）来控制实验室饲养的弓背蚁菌落中细菌内共生菌的数量。使用布洛曼尼亚特异性引物（qPCR）和两种荧光探针（一种用于所有真细菌，另一种针对布洛曼尼亚），通过定量聚合酶链反应和荧光原位杂交（FISH）来评估治疗效率。在经过处理的蚂蚁中检测到很少或根本没有细菌。初始利福平处理的群体的育雏和成年工蚁数量显着低于对照群体。通过将尼龙丝插入胃中并在 24 小时后将其取出来评估来自对照和处理群体的蚂蚁的免疫反应。在对照菌落中，包囊反应与细菌量呈正相关，而在处理菌落中没有观察到相关性。事实上，抗生素治疗增加了工蚁的封装反应，这可能是由于压力条件所致。在未经处理的菌落中观察到的生长速度增加证实了布洛曼尼亚在菌落发育的这一阶段的重要性。这将在菌落建立过程中提供重要的选择优势，此时菌落面临着激烈的种间和种内竞争。此外，细菌改善了工人的封装反应。因此，这些蚂蚁可能不太容易受到各种病原体的攻击，例如通常在弓背蚁巢附近发现的拟蝇科寄生蜂。这些优势或许可以解释这个由 1000 多个物种组成的蚂蚁属在生态方面取得的显着成功。
Microorganisms are a large and diverse form of life. Many of them live in association with large multicellular organisms, developing symbiotic relations with the host and some have even evolved to form obligate endosymbiosis. All Carpenter ants (genus Camponotus) studied hitherto harbour primary endosymbiotic bacteria of the Blochmannia genus. The role of these bacteria in ant nutrition has been demonstrated but the omnivorous diet of these ants lead us to hypothesize that the bacteria might provide additional advantages to their host. In this study, we establish links between Blochmannia, growth of starting new colonies and the host immune response. We manipulated the number of bacterial endosymbionts in incipient laboratory-reared colonies of Camponotus fellah by administrating doses of an antibiotic (Rifampin) mixed in honey-solution. Efficiency of the treatment was estimated by quantitative polymerase chain reaction and Fluorescent in situ hybridization (FISH), using Blochmannia specific primers (qPCR) and two fluorescent probes (one for all Eubacterial and other specific for Blochmannia). Very few or no bacteria could be detected in treated ants. Incipient Rifampin treated colonies had significantly lower numbers of brood and adult workers than control colonies. The immune response of ants from control and treated colonies was estimated by inserting nylon filaments in the gaster and removing it after 24 h. In the control colonies, the encapsulation response was positively correlated to the bacterial amount, while no correlation was observed in treated colonies. Indeed, antibiotic treatment increased the encapsulation response of the workers, probably due to stress conditions. The increased growth rate observed in non-treated colonies confirms the importance of Blochmannia in this phase of colony development. This would provide an important selective advantage during colony founding, where the colonies are faced with severe inter and intraspecific competition. Furthermore, the bacteria improve the workers encapsulation response. Thus, these ants are likely to be less susceptible to various pathogen attacks, such as the Phoridae fly parasitoids, normally found in the vicinity of Camponotus nests. These advantages might explain the remarkable ecological success of this ant genus, comprising more than 1000 species.
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DOI：
10.1073/pnas.0611659104
发表时间：
2007-05-15
影响因子：
11.1
作者：
Moran, Nancy A.
通讯作者：
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DOI：
10.1098/rspb.2005.3383
发表时间：
2006-04-07
影响因子：
4.7
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DOI：
10.1073/pnas.101304298
发表时间：
2001-05-22
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
7
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DOI：
10.1073/pnas.1533499100
发表时间：
2003-08-05
影响因子：
11.1
作者：
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