Fibrin architecture in clots: a quantitative polarized light microscopy analysis.
Fibrin architecture in clots: a quantitative polarized light microscopy analysis.
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DOI：
10.1016/j.bcmd.2008.10.014
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发表时间：
2009-01
影响因子：
2.3
通讯作者：
Przyklenk, Karin
中科院分区：
文献类型：
--
作者：
Whittaker, Peter;Przyklenk, Karin
纤维蛋白酶在血栓完整性中起着至关重要的结构作用。因此，评估纤维蛋白结构的能力有可能提供对血栓形成和血栓溶解的了解。纤维素具有各向异性的分子结构，这使得它能够用偏振光看到。因此，我们的目的是确定是否可以使用自动偏振光显微镜方法定量两个结构参数;纤维蛋白纤维束取向和纤维蛋白的光学延迟（OR：分子各向异性的量度）来评估血栓。为了比较纤维蛋白纤维束方向，我们分析了从以下形成的凝块中获得的天狼星红染色切片：（A）体外，（B）损伤和狭窄的冠状动脉，和（C）手术创建的主动脉瘤（每组n = 6）。为了评估OR的潜在变化，我们检查了缺血预处理（10分钟缺血+10分钟再流;显示增强溶解性的情况）后形成的天狼星红染色凝块中的纤维蛋白和对照凝块中的纤维蛋白（每组n = 8）。根据凝块形成的位置，纤维蛋白组织化的程度显著不同;纤维蛋白在动脉瘤中排列最多，在体外排列最少，而冠状动脉凝块中的纤维蛋白具有中间组织。缺血预处理组血栓中纤维蛋白的比值比（OR）低于对照组（2.9 ± 0.5nmvs5.4 ± 1.0nm，P < 0.05）。自动偏振光分析方法不仅能够评估纤维蛋白结构，而且还揭示了在不同情况下形成的凝块的结构差异。
Fibrin plays a vital structural role in thrombus integrity. Thus, the ability to assess fibrin architecture has potential to provide insight into thrombosis and thrombolysis. Fibrin has an anisotropic molecular structure, which enables it to be seen with polarized light. Therefore, we aimed to determine if automated polarized light microscopy methods of quantifying two structural parameters; fibrin fiber bundle orientation and fibrin's optical retardation (OR: a measure of molecular anisotropy) could be used to assess thrombi. To compare fibrin fiber bundle orientation we analyzed picrosirius red-stained sections obtained from clots formed: (A) in vitro, (B) in injured and stenotic coronary arteries, and (C) in surgically created aortic aneurysms (n = 6 for each group). To assess potential changes in OR, we examined fibrin in picrosirius red-stained clots formed after ischemic preconditioning (10 minutes ischemia + 10 minutes reflow; a circumstance shown to enhance lysability) and in control clots (n = 8 each group). The degree of fibrin organization differed significantly according to the location of clot formation; fibrin was most aligned in the aneurysms and least aligned in vitro whereas fibrin in the coronary clots had an intermediate organization. The OR of fibrin in the clots formed after ischemic preconditioning was lower than that in controls (2.9 ± 0.5 nm versus 5.4 ± 1.0 nm, P < 0.05). The automated polarized light analysis methods not only enabled fibrin architecture to be assessed, but also revealed structural differences in clots formed under different circumstances.
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