Antibacterial Activity of Protocatechuic Acid Ethyl Ester on Staphylococcus aureus Clinical Strains Alone and in Combination with Antistaphylococcal Drugs.
Antibacterial Activity of Protocatechuic Acid Ethyl Ester on Staphylococcus aureus Clinical Strains Alone and in Combination with Antistaphylococcal Drugs.
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DOI：
10.3390/molecules200813536
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发表时间：
2015-07-23
期刊：
影响因子：
--
通讯作者：
Wąsik TJ
中科院分区：
文献类型：
--
作者：
Miklasińska M;Kępa M;Wojtyczka RD;Idzik D;Zdebik A;Orlewska K;Wąsik TJ
本研究的目的是研究原儿茶酸乙酯（乙基3,4-二羟基苯甲酸乙酯，EDHB）对金黄色葡萄球菌临床分离株的体外抗菌活性。研究了EDHB对临床分离的20株金黄色葡萄球菌和3株参考菌株的抑菌活性。测定了各菌株对甲氧西林的耐药表型和基因型，以及对大环内酯类、林肯胺类和链状gramin B （MLSB）的耐药表型。EDHB对金黄色葡萄球菌具有不同的抑制活性，最小抑制浓度（MIC）在64 ~ 1024µg/mL之间。将1 / 4 MIC的EDHB加入到muller - hinton琼脂（MHA）中，在克林霉素存在的情况下，抗菌效果增强。在头孢西丁的情况下，没有发现与EDHB的协同作用。红霉素和万古霉素在EDHB存在时平均mic下降，但未达到统计学意义。本研究结果表明，EDHB在体外对金黄色葡萄球菌临床菌株具有抑菌活性，与克林霉素有协同抑菌作用，与红霉素、万古霉素有较弱的协同抑菌作用。
The aim of the presented study was to examine in vitro the antibacterial activity of protocatechuic acid ethyl ester (ethyl 3,4-dihydroxybenzoate, EDHB) against Staphylococcus aureus clinical isolates alone and in the combination with four selected antibiotics. The EDHB antimicrobial activity was tested against twenty S. aureus strains isolated from the clinical samples, and three reference strains. The phenotypes and genotypes of resistance to methicillin for the tested strains were defined as well as the phenotypic resistance to macrolides, lincosamides and streptogramin B (MLSB). EDHB displayed diverse activity against examined S. aureus strains with the minimal inhibitory concentration (MIC) within the range from 64 to 1024 µg/mL. Addition of ¼ MIC of EDHB into the Mueller-Hinton Agar (MHA) resulted in augmented antibacterial effect in the presence of clindamycin. In the case of cefoxitin no synergistic effect with EDHB was noted. For erythromycin and vancomycin the decrease of mean MICs in the presence of EDHB was observed but did not reach statistical significance. The results of the present study showed that in vitro EDHB possesses antibacterial activity against S. aureus clinical strains and triggers a synergistic antimicrobial effect with clindamycin and to the lesser extent with erythromycin and vancomycin.
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影响因子：
11.2
作者：
Gilkes DM;Chaturvedi P;Bajpai S;Wong CC;Wei H;Pitcairn S;Hubbi ME;Wirtz D;Semenza GL
通讯作者：
Semenza GL
脯氨酰羟化酶抑制剂 3,4-二羟基苯甲酸乙酯通过上调 BNIP3 和 N-myc 下游调节基因 1 诱导食管鳞状细胞癌细胞自噬和凋亡
影响因子：
3.7
作者：
Han B;Li W;Sun Y;Zhou L;Xu Y;Zhao X
通讯作者：
Zhao X
影响因子：
5.9
作者：
Kang SN;Lee JS;Park JH;Cho JH;Park JH;Cho KK;Lee OH;Kim IS
通讯作者：
Kim IS
影响因子：
2.8
作者：
Chao, Che-Yi;Yin, Mei-Chin
通讯作者：
Yin, Mei-Chin
影响因子：
5.4
作者：
Kuete, Victor;Nana, Frederic;Ngadjui, Bonaventure Tchaleu
通讯作者：
Ngadjui, Bonaventure Tchaleu