Complete genome analysis of three Acinetobacter baumannii clinical isolates in China for insight into the diversification of drug resistance elements.
Complete genome analysis of three Acinetobacter baumannii clinical isolates in China for insight into the diversification of drug resistance elements.
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DOI：
10.1371/journal.pone.0066584
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Li QZ
中科院分区：
文献类型：
--
作者：
Zhu L;Yan Z;Zhang Z;Zhou Q;Zhou J;Wakeland EK;Fang X;Xuan Z;Shen D;Li QZ
多重耐药鲍曼不动杆菌菌株的出现和迅速传播已成为全世界的主要健康威胁。为了更好地了解细菌感染过程中与耐药元件获得相关的基因重组，我们利用二代测序技术对北京新分离的三株多重耐药鲍曼不动杆菌进行了完整的基因组分析。全基因组比较显示，3个菌株具有一些共同的耐药元件，包括碳青霉烯类耐药bla OXA-23和四环素（tet）耐药岛，但菌株之间的基因组结构存在差异。各种基因组岛通过转座子和插入散布在基因组上，反映了获得抗性元件期间的重组灵活性。血液分离的BJAB07104和腹水分离的BJAB0868在基因组结构上与大多数全球克隆II菌株表现出高度相似性，表明这两种菌株属于全球流行的优势爆发菌株。 BJAB07104 和 BJAB0868 基因组上的 ATPase 基因中插入了一个约 121 kb 的大抗性岛 (RI)，携带一簇抗性相关基因。携带 tra 位点和 bla OXA-23 岛的 78 kb 插入元件可以插入染色体上 121 kb RI 中的 tniB 基因之一，也可以转化为两个 BJAB 菌株中的接合质粒。本研究的第三个菌株，BJAB0715，是从脊髓液中分离出来的，与上述两个菌株相比，表现出更大的差异。它含有多种耐药元件，包括其基因组上截短的 AbaR-22 样 RI。该菌株的独特特征之一是其基因组上同时携带 bla OXA-23 和 bla OXA-58 基因。此外，还发现鲁氏不动杆菌adeABC外排元件插入BJAB0715的ATPase位置。我们对目前完成的鲍曼不动杆菌基因组的比较分析揭示了广泛且动态的基因组组织，这可能有助于细菌将耐药元件获取到其基因组中。
The emergence and rapid spreading of multidrug-resistant Acinetobacter baumannii strains has become a major health threat worldwide. To better understand the genetic recombination related with the acquisition of drug-resistant elements during bacterial infection, we performed complete genome analysis on three newly isolated multidrug-resistant A. baumannii strains from Beijing using next-generation sequencing technology. Whole genome comparison revealed that all 3 strains share some common drug resistant elements including carbapenem-resistant bla OXA-23 and tetracycline (tet) resistance islands, but the genome structures are diversified among strains. Various genomic islands intersperse on the genome with transposons and insertions, reflecting the recombination flexibility during the acquisition of the resistant elements. The blood-isolated BJAB07104 and ascites-isolated BJAB0868 exhibit high similarity on their genome structure with most of the global clone II strains, suggesting these two strains belong to the dominant outbreak strains prevalent worldwide. A large resistance island (RI) of about 121-kb, carrying a cluster of resistance-related genes, was inserted into the ATPase gene on BJAB07104 and BJAB0868 genomes. A 78-kb insertion element carrying tra-locus and bla OXA-23 island, can be either inserted into one of the tniB gene in the 121-kb RI on the chromosome, or transformed to conjugative plasmid in the two BJAB strains. The third strains of this study, BJAB0715, which was isolated from spinal fluid, exhibit much more divergence compared with above two strains. It harbors multiple drug-resistance elements including a truncated AbaR-22-like RI on its genome. One of the unique features of this strain is that it carries both bla OXA-23 and bla OXA-58 genes on its genome. Besides, an Acinetobacter lwoffii adeABC efflux element was found inserted into the ATPase position in BJAB0715. Our comparative analysis on currently completed Acinetobacter baumannii genomes revealed extensive and dynamic genome organizations, which may facilitate the bacteria to acquire drug-resistance elements into their genomes.
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