Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer.
Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer.
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DOI：
10.3791/63086
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发表时间：
2022-06-10
期刊：
影响因子：
--
通讯作者：
Maguire A
中科院分区：
文献类型：
--
作者：
Wisner L;Larsen B;Maguire A
组织微阵列（TMA）是一种重要的研究工具，它可以在一个石蜡块中代表许多福尔马林固定石蜡包埋（FFPE）的样本。这是通过使用从不同供体FFPE块的感兴趣区域提取的组织芯并将它们排列成单个TMA石蜡块来实现的。一旦构建完成，来自完整TMA的切片可用于进行免疫组织化学、显色、荧光原位杂交（FISH）和RNA ISH研究，以同时评估许多样品中的蛋白质表达以及基因组和转录改变，从而最大限度地减少组织使用并降低试剂成本。有几种不同的TMA施工技术。最常见的施工方法之一是接收器方法，该方法最适用于相同长度的芯，建议最小长度为4 mm。不幸的是，在诊断过程中，组织块可能会被大量切除，经常导致厚度小于4 mm的“非理想”供体块。当前的文章和视频集中在双面胶带方法上;一种替代的手动、低成本、易于使用和快速的方法来构建与这些非理想供体块高度兼容的低密度（<50个核心）TMA。该方案提供了如何使用该方法构建TMA的分步指南，重点关注病理审查和构建后验证的至关重要性。该方案概述了如何使用不同深度的FFPE供体块手动构建组织微阵列的胶带方法。
The tissue microarray (TMA) is an important research tool in which many formalin fixed paraffin embedded (FFPE) samples can be represented in a single paraffin block. This is achieved by using tissue cores extracted from the region of interest of different donor FFPE blocks and arranging them into a single TMA paraffin block. Once constructed, sections from the completed TMA can be used to perform immunohistochemistry, chromogenic, fluorescence in situ hybridization (FISH) and RNA ISH studies to assess protein expression as well as genomic and transcriptional alterations in many samples simultaneously, thus minimizing tissue usage and reducing reagent costs. There are several different TMA construction techniques. One of the most common construction methods is the recipient method which works best with cores of the same length for which a minimum length of 4 mm is recommended. Unfortunately, tissue blocks can be heavily resected during the diagnostic process, frequently resulting in “non-ideal” donor block thicknesses of less than 4 mm. The current article and video focuses on the double-sided adhesive tape method; an alternative manual, low cost, easy to use and rapid method to construct low density (<50 cores) TMAs that is highly compatible with these non-ideal donor blocks. This protocol provides a step-by-step guide on how to construct a TMA using this method, with a focus on the critical importance of pathological review and post construction validation. This protocol outlines the tape method on how to manually construct a tissue microarray using FFPE donor blocks of differing depths.
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发表时间：
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期刊：
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影响因子：
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