Mechanisms of neutrophil recruitment to the lung by grain dust exposure.
Mechanisms of neutrophil recruitment to the lung by grain dust exposure.
复制标题
谷物粉尘暴露导致中性粒细胞募集至肺部的机制。
DOI：
10.1164/ajrccm/138.4.921
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
S. Rennard
中科院分区：
文献类型：
--
作者：
S. V. Essen;R. Robbins;Austin B. Thompson;R. Ertl;J. Linder;J. Linder;S. Rennard
吸入颗粒粉尘可引起急性和慢性支气管炎的症状，这与肺部的中性粒细胞炎症反应有关。由于这种中性粒细胞的涌入可能导致支气管损伤，因此研究了中性粒细胞向颗粒粉尘募集的机制。无菌的、含水的谷物粉尘提取物是由谷物高粱、玉米、燕麦和大豆的沉淀粉尘制备的。使用盲孔中性粒细胞趋化试验评估提取物直接吸引人中性粒细胞的能力。与对照组相比，各提取物均具有显著的趋化活性（p < 0.01）。为了评估粉尘是否可以通过激活体液炎症机制间接吸引中性粒细胞，我们评估了颗粒粉尘提取物激活补体系统的能力。当与正常人血清孵育时，每种颗粒粉尘都引起补体蛋白C3和适当的蛋白因子B （PFB）的裂解。重要的是，颗粒粉尘提取物导致C5a的产生，C5a是补体激活产生的一种有效的中性粒细胞化学引诱剂。由于激活肺泡巨噬细胞释放趋化活性是中性粒细胞募集的另一种间接机制，因此我们评估了高粱粉提取物刺激豚鼠和人肺泡巨噬细胞释放中性粒细胞趋化活性的能力。结果表明，与对照组相比，高粱粉提取物培养的肺泡巨噬细胞上清具有更高的趋化活性（p < 0.01）。（摘要删节250字）
Inhalation of grain dusts can cause symptoms of both acute and chronic bronchitis, which has been associated with a neutrophilic inflammatory response in the lung. Since this influx of neutrophils may potentially result in bronchial injury, mechanisms of neutrophil recruitment to grain dust were examined. Sterile, aqueous grain dust extracts were prepared from settled dusts of grain sorghum, corn, oats, and soybeans. The extracts were evaluated for their ability to directly attract human neutrophils using a blindwell neutrophil chemotaxis assay. Each extract was found to possess significant chemotactic activity compared to control (p less than 0.01). To evaluate if the dusts could attract neutrophils indirectly by activating humoral inflammatory mechanisms, the grain dust extracts were evaluated for their ability to activate the complement system. When incubated with normal human serum, each of the grain dusts caused cleavage of the complement proteins C3 and properdin factor B (PFB). Importantly, the grain dust extracts lead to the generation of C5a, a potent neutrophil chemoattractant generated by complement activation. Since activation of the alveolar macrophage to release chemotactic activity represents an additional indirect mechanism of neutrophil recruitment, an extract from grain sorghum dust was evaluated for its ability to stimulate guinea pig and human alveolar macrophages to release neutrophil chemotactic activity. The results demonstrate that supernatants obtained from alveolar macrophages cultured in the presence of grain sorghum dust extract possessed increased chemotactic activity compared to controls (p less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
人肺泡巨噬细胞产生白三烯 B4：放大肺部炎症的潜在机制。
DOI：
10.1164/arrd.1984.129.1.106
发表时间：
1984
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Martin,TR;Altman,LC;Albert,RK;Henderson,WR
通讯作者：
Henderson,WR
成人呼吸窘迫综合征患者支气管肺泡灌洗液中的中性粒细胞弹性蛋白酶释放因子。
DOI：
10.1164/arrd.1985.132.5.1098
发表时间：
1985
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Idell,S;Kucich,U;Fein,A;Kueppers,F;James,HL;Walsh,PN;Weinbaum,G;Colman,RW;Cohen,AB
通讯作者：
Cohen,AB