Transcriptional responses of winter barley to cold indicate nucleosome remodelling as a specific feature of crown tissues.
Transcriptional responses of winter barley to cold indicate nucleosome remodelling as a specific feature of crown tissues.
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DOI：
10.1007/s10142-011-0213-8
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发表时间：
2011-06
影响因子：
2.9
通讯作者：
Ovesna, Jaroslava
中科院分区：
文献类型：
--
作者：
Janska, Anna;Aprile, Alessio;Zamecnik, Jiri;Cattivelli, Luigi;Ovesna, Jaroslava
我们报道了一系列基于微阵列的冬季大麦品种Luxor的叶片和冠的基因表达比较，在幼苗暴露于不同时期的低温（高于零度和低于零度）之后。转录组学分析确定了在叶和冠中都表达的基因，或者只在其中一个或另一个中表达。前者主要负责钙和脱落酸信号、多胺合成、胚胎发育晚期、丰富的蛋白质和脱水剂。在谷物越冬的关键器官冠部，低温处理导致核小体组装基因转录的短暂变化，尤其是与拟南芥冷感知有关的H2A和HTA11。在叶片中，诱导了多种热休克蛋白。涉及渗透产物（蔗糖和淀粉、棉子糖、γ-氨基丁酸代谢）、糖信号（海藻糖代谢）和次生代谢（木质素合成）的某些途径的基因在树冠和叶片之间的表达模式经常存在差异。具有抗冻活性的蛋白质在硬化过程中被显著诱导，其作用通过冰成核温度的降低得到证实。本文的在线版本（doi:10.1007/s10142-011-0213-8）包含补充材料，仅供授权用户使用。
We report a series of microarray-based comparisons of gene expression in the leaf and crown of the winter barley cultivar Luxor, following the exposure of young plants to various periods of low (above and below zero) temperatures. A transcriptomic analysis identified genes which were either expressed in both the leaf and crown, or specifically in one or the other. Among the former were genes responsible for calcium and abscisic acid signalling, polyamine synthesis, late embryogenesis abundant proteins and dehydrins. In the crown, the key organ for cereal overwintering, cold treatment induced transient changes in the transcription of nucleosome assembly genes, and especially H2A and HTA11, which have been implicated in cold sensing in Arabidopsis thaliana. In the leaf, various heat-shock proteins were induced. Differences in expression pattern between the crown and leaf were frequent for genes involved in certain pathways responsible for osmolyte production (sucrose and starch, raffinose, γ-aminobutyric acid metabolism), sugar signalling (trehalose metabolism) and secondary metabolism (lignin synthesis). The action of proteins with antifreeze activity, which were markedly induced during hardening, was demonstrated by a depression in the ice nucleation temperature. The online version of this article (doi:10.1007/s10142-011-0213-8) contains supplementary material, which is available to authorized users.
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影响因子：
3.1
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