Modelling central metabolic fluxes by constraint-based optimization reveals metabolic reprogramming of developing Solanum lycopersicum (tomato) fruit.
Modelling central metabolic fluxes by constraint-based optimization reveals metabolic reprogramming of developing Solanum lycopersicum (tomato) fruit.
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DOI：
10.1111/tpj.12685
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发表时间：
2015-01
期刊：
影响因子：
--
通讯作者：
Gibon Y
中科院分区：
文献类型：
--
作者：
Colombié S;Nazaret C;Bénard C;Biais B;Mengin V;Solé M;Fouillen L;Dieuaide-Noubhani M;Mazat JP;Beauvoit B;Gibon Y
代谢网络的建模是分析包括果实在内的植物器官发育行为的有力工具。根据我们目前对植物细胞异养代谢的理解，构建了一个中等尺度的化学计量模型，包括辅助因子和能量的平衡，以描述在茄茄果实发育的九个连续阶段中发生的代谢变化。将每一阶段测定的主要生物量成分和果皮中积累的代谢物的测量浓度进行拟合，通过推导计算相应的外部通量。将它们作为约束，通过最小化内部通量来求解模型。然后分析计算的中心代谢通量的分布，并与已知的代谢行为进行比较。例如，主要代谢途径（糖酵解、戊糖磷酸途径等）的分配与整个果实发育有关。我们还预测了水果对碳和氮的有效进口，以及一致的二氧化碳释放。有趣的是，能量平衡表明，过量的ATP在成熟开始之前就消散了，这支持了更年期危机的概念。最后，计算出的通量与测量到的酶容量之间的明显矛盾表明，在果实发育过程中，代谢机制发生了复杂的重编程。通过对代谢系统的精确定义和强有力的实验数据，本工作为了解发育中的番茄果实的代谢和生理需求提供了重要的见解。
Modelling of metabolic networks is a powerful tool to analyse the behaviour of developing plant organs, including fruits. Guided by our current understanding of heterotrophic metabolism of plant cells, a medium-scale stoichiometric model, including the balance of co–factors and energy, was constructed in order to describe metabolic shifts that occur through the nine sequential stages of Solanum lycopersicum (tomato) fruit development. The measured concentrations of the main biomass components and the accumulated metabolites in the pericarp, determined at each stage, were fitted in order to calculate, by derivation, the corresponding external fluxes. They were used as constraints to solve the model by minimizing the internal fluxes. The distribution of the calculated fluxes of central metabolism were then analysed and compared with known metabolic behaviours. For instance, the partition of the main metabolic pathways (glycolysis, pentose phosphate pathway, etc.) was relevant throughout fruit development. We also predicted a valid import of carbon and nitrogen by the fruit, as well as a consistent CO2 release. Interestingly, the energetic balance indicates that excess ATP is dissipated just before the onset of ripening, supporting the concept of the climacteric crisis. Finally, the apparent contradiction between calculated fluxes with low values compared with measured enzyme capacities suggest a complex reprogramming of the metabolic machinery during fruit development. With a powerful set of experimental data and an accurate definition of the metabolic system, this work provides important insight into the metabolic and physiological requirements of the developing tomato fruits.
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影响因子：
6.1
作者：
HOEBLER, C;BARRY, JL;DELORTLAVAL, J
通讯作者：
DELORTLAVAL, J
影响因子：
5.1
作者：
Bahrami, AR;Chen, ZH;Gray, JE
通讯作者：
Gray, JE
影响因子：
7.4
作者：
Dal'Molin, Cristiana Gomes de Oliveira;Quek, Lake-Ee;Nielsen, Lars Keld
通讯作者：
Nielsen, Lars Keld
影响因子：
3.1
作者：
BLAKENEY, AB;HARRIS, PJ;STONE, BA
通讯作者：
STONE, BA
影响因子：
7.2
作者：
Hay, Jordan;Schwender, Joerg
通讯作者：
Schwender, Joerg