Revolver is a new class of transposon-like gene composing the triticeae genome.
Revolver is a new class of transposon-like gene composing the triticeae genome.
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DOI：
10.1093/dnares/dsm029
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发表时间：
2008-02-29
期刊：
影响因子：
--
通讯作者：
Morimoto M
中科院分区：
文献类型：
--
作者：
Tomita M;Shinohara K;Morimoto M
在小麦科植物中发现的 Revolver 是一类新型的转座子样基因，也是大型谷类基因组的主要组成部分。通过删除黑麦和小麦共有的 DNA 序列，分离出富含黑麦基因组的 Revolver 89 bp 片段。 Revolver 的整个结构是通过使用黑麦基因组克隆确定的，并通过 89 bp 探针进行筛选。 Revolver 由 2929-3041 bp 组成，两端各有一个反向重复序列，分散在黑麦基因组的所有 7 条染色体中。 Revolver具有转录活性，分离的全长cDNA（726 bp）显示，Revolver含有一个由三个外显子（342、88和296 bp）和两个内含子（750和1237 bp）组成的单一基因，编码139个氨基酸残基的蛋白质，与一些转录调节因子相似。 Revolver 变体范围为 2665 至 4269 bp，其中 5' 区域被破坏，表明第一个外显子周围存在结构多样性。 Revolver 不与任何活物种的任何已知的 I 类或 II 类自主转座元件共享同一性。对小麦科植物的 DNA 印迹分析表明，Revolver 自小麦的二倍体祖先以来就已存在，并且在小麦科的进化过程中在多个物种中被扩增或丢失。
Revolver discovered in the Triticeae plant is a novel class of transposon-like gene and a major component of the large cereal genome. An 89 bp segment of Revolver that is enriched in the genome of rye was isolated by deleting the DNA sequences common to rye and wheat. The entire structure of Revolver was determined by using rye genomic clones, which were screened by the 89 bp probe. Revolver consists of 2929—3041 bp with an inverted repeated sequence on each end and is dispersed through all seven chromosomes of the rye genome. Revolver is transcriptionally active, and the isolated full-length cDNA (726 bp) reveals that Revolver harbors a single gene consisting of three exons (342, 88, and 296 bp) and two introns (750 and 1237 bp), and encodes 139 amino acid residues of protein, which shows similarity to some transcriptional regulators. Revolver variants ranging from 2665 to 4269 bp, in which 5′ regions were destructed, indicate structural diversities around the first exon. Revolver does not share identity with any known class I or class II autonomous transposable elements of any living species. DNA blot analysis of Triticeae plants shows that Revolver has existed since the diploid progenitor of wheat, and has been amplified or lost in several species during the evolution of the Triticeae.
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