The Thiamine diphosphate dependent Enzyme Engineering Database: a tool for the systematic analysis of sequence and structure relations.
The Thiamine diphosphate dependent Enzyme Engineering Database: a tool for the systematic analysis of sequence and structure relations.
复制标题
DOI：
10.1186/1471-2091-11-9
复制
发表时间：
2010-02-01
期刊：
影响因子：
--
通讯作者：
Pleiss J
中科院分区：
文献类型：
--
作者：
Widmann M;Radloff R;Pleiss J
依赖于硫胺素二磷酸(ThDP)的酶形成了一大类不同的蛋白质，催化了各种各样的酶反应，包括碳-硫、碳-氧、碳-氮的形成或裂解，特别是碳-碳键的形成或断裂。虽然它们在序列和结构域组织上非常不同，但它们共享两个共同的蛋白质结构域，焦磷酸(PP)和嘧啶(PYR)结构域。为了对蛋白质序列和结构进行全面和系统的比较，建立了硫胺二磷酸依赖的酶工程数据库(TEED)。TEED http://www.teed.uni-stuttgart.de包含12048个序列条目，分别属于9,443个不同的蛋白质和379个结构条目。蛋白质分为8个不同的超家族和63个同源蛋白质家族。对于每个家族，TED提供了多序列比对、系统发育树和特定于家族的HMM配置文件。保守的焦磷酸(PP)和嘧啶(PYR)结构域已经被注释，这使得分析各种蛋白质的序列相似性成为可能。人类ThDP依赖的酶被认为与许多疾病有关。在TED中发现了20种不同的蛋白质和40多个人类ThDP依赖酶的单核苷酸多态(SNPs)。Ted的在线可访问版本被设计为ThDP依赖酶的大型和多样化家族的导航和分析工具。
Thiamine diphosphate (ThDP)-dependent enzymes form a vast and diverse class of proteins, catalyzing a wide variety of enzymatic reactions including the formation or cleavage of carbon-sulfur, carbon-oxygen, carbon-nitrogen, and especially carbon-carbon bonds. Although very diverse in sequence and domain organisation, they share two common protein domains, the pyrophosphate (PP) and the pyrimidine (PYR) domain. For the comprehensive and systematic comparison of protein sequences and structures the Thiamine diphosphate (ThDP)-dependent Enzyme Engineering Database (TEED) was established. The TEED http://www.teed.uni-stuttgart.de contains 12048 sequence entries which were assigned to 9443 different proteins and 379 structure entries. Proteins were assigned to 8 different superfamilies and 63 homologous protein families. For each family, the TEED offers multisequence alignments, phylogenetic trees, and family-specific HMM profiles. The conserved pyrophosphate (PP) and pyrimidine (PYR) domains have been annotated, which allows the analysis of sequence similarities for a broad variety of proteins. Human ThDP-dependent enzymes are known to be involved in many diseases. 20 different proteins and over 40 single nucleotide polymorphisms (SNPs) of human ThDP-dependent enzymes were identified in the TEED. The online accessible version of the TEED has been designed to serve as a navigation and analysis tool for the large and diverse family of ThDP-dependent enzymes.
登录
查看更多内容
影响因子：
3
作者：
Fischer M;Thai QK;Grieb M;Pleiss J
通讯作者：
Pleiss J
影响因子：
14.9
作者：
Benson DA;Karsch-Mizrachi I;Lipman DJ;Ostell J;Sayers EW
通讯作者：
Sayers EW
影响因子：
14.9
作者：
Fischer, M;Pleiss, J
通讯作者：
Pleiss, J
影响因子：
14.9
作者：
Bairoch, Amos;Bougueleret, Lydie;Zhang, Jian
通讯作者：
Zhang, Jian
DOI：
10.1107/s0907444907050433
发表时间：
2007-12-01
影响因子：
2.2
作者：
Berthold, Catrine L.;Gocke, Doerte;Schneider, Gunter
通讯作者：
Schneider, Gunter