Role of SFP1 in the Regulation of Candida albicans Biofilm Formation.
Role of SFP1 in the Regulation of Candida albicans Biofilm Formation.
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DOI：
10.1371/journal.pone.0129903
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Lan CY
中科院分区：
文献类型：
--
作者：
Chen HF;Lan CY
白色念珠菌是一种主要的人类真菌病原体。C.白色念珠菌的致病性是在粘膜表面和留置医疗装置上形成生物膜的能力。生物被膜的形成涉及复杂的过程。包括细胞粘附、丝状生长、细胞外基质分泌和细胞分散。在这项工作中，我们的特点是转录因子Sfp 1的作用，特别是其功能的生物膜形成的调节。与野生型菌株相比，SFP 1基因的缺失增强了细胞粘附和生物膜形成。有趣的是，sfp 1缺失突变体也表现出编码粘附素蛋白的ALS 1、ALS 3和HWP 1基因表达的增加。此外，Sfp 1被证明在Rhb 1-TOR信号通路的下游发挥作用。Bcr 1和Efg 1是控制生物膜形成的关键转录因子，Efg 1也是菌丝生长所必需的。在sfp 1-null背景中删除BCR 1或EFG 1基因导致粘附素基因表达减少。结果，bcr 1/sfp 1或efg 1/sfp 1双缺失突变体表现出显著减少的生物膜形成。结果表明，Sfp 1负调节ALS 1、ALS 3和HWP 1粘附素基因，并且这些基因的抑制是通过抑制Bcr 1和Efg 1介导的。
Candida albicans is a major human fungal pathogen. One of the important features of C. albicans pathogenicity is the ability to form biofilms on mucosal surfaces and indwelling medical devices. Biofilm formation involves complex processes in C. albicans, including cell adhesion, filamentous growth, extracellular matrix secretion and cell dispersion. In this work, we characterized the role of the transcription factor Sfp1, particularly with respect to its function in the regulation of biofilm formation. The deletion of the SFP1 gene enhanced cell adhesion and biofilm formation in comparison to the wild-type strain. Interestingly, the sfp1-deleted mutant also exhibited an increase in the expression of the ALS1, ALS3 and HWP1 genes, which encode adhesin proteins. In addition, Sfp1 was demonstrated to function downstream of the Rhb1-TOR signaling pathway. Bcr1 and Efg1 are transcription factors that are critical for controlling biofilm formation, and Efg1 is also required for hyphal growth. Deleting either the BCR1 or EFG1 gene in the sfp1-null background led to reduced adhesin gene expression. As a result, the bcr1/sfp1 or efg1/sfp1 double deletion mutants exhibited dramatically reduced biofilm formation. The results indicated that Sfp1 negatively regulates the ALS1, ALS3 and HWP1 adhesin genes and that the repression of these genes is mediated by the inhibition of Bcr1 and Efg1.
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影响因子：
6.7
作者：
Lin CH;Kabrawala S;Fox EP;Nobile CJ;Johnson AD;Bennett RJ
通讯作者：
Bennett RJ
影响因子：
3
作者：
Baillie, GS;Douglas, LJ
通讯作者：
Douglas, LJ
影响因子：
--
作者：
Hsu, Po-Chen;Chao, Chun-Cheih;Lan, Chung-Yu
通讯作者：
Lan, Chung-Yu
影响因子：
3
作者：
Kucharikova, Sona;Tournu, Helene;Bujdakova, Helena
通讯作者：
Bujdakova, Helena
影响因子：
3.1
作者：
Andes, D;Nett, J;Pitula, A
通讯作者：
Pitula, A