Plant flavones enhance antimicrobial activity of respiratory epithelial cell secretions against Pseudomonas aeruginosa.
Plant flavones enhance antimicrobial activity of respiratory epithelial cell secretions against Pseudomonas aeruginosa.
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DOI：
10.1371/journal.pone.0185203
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Lee RJ
中科院分区：
文献类型：
--
作者：
Hariri BM;McMahon DB;Chen B;Adappa ND;Palmer JN;Kennedy DW;Lee RJ
黄酮类化合物是一类具有抗炎、抗菌作用的天然植物次生代谢产物。一些黄酮还激活T2R14苦味受体，其在鼻窦上皮的运动纤毛中表达并激活先天免疫一氧化氮（NO）产生。因此，黄酮类化合物可能是呼吸道感染的潜在治疗剂。我们的目的是研究黄酮对常见的鼻窦病原体白色念珠菌、金黄色葡萄球菌和铜绿假单胞菌的抗菌作用，评估其抑菌和生物膜生长。黄酮类化合物只有非常低水平的抗菌活性。它们不减少生物膜形成，但减少重要的铜绿假单胞菌炎症介质和纤毛毒素绿脓菌素的产生。然而，黄酮类化合物在抗生素或重组人溶菌酶的存在下对铜绿假单胞菌表现出协同作用。它们还增强了在气液界面生长的培养的和原代人气道细胞分泌的抗菌剂的功效。这表明，黄酮类化合物与抗生素联合使用或与呼吸道或其他上皮细胞分泌的先天性抗菌剂联合使用时，可能具有抗革兰氏阴性菌的潜力。当与T2R14激活的NO产生结合时，这可能具有累加效应。进一步的研究是必要的，以了解哪些黄酮化合物或混合物是最有效的。
Flavones are a class of natural plant secondary metabolites that have anti-inflammatory and anti-bacterial effects. Some flavones also activate the T2R14 bitter taste receptor, which is expressed in motile cilia of the sinonasal epithelium and activates innate immune nitric oxide (NO) production. Flavones may thus be potential therapeutics for respiratory infections. Our objective was to examine the anti-microbial effects of flavones on the common sinonasal pathogens Candida albicans, Staphylococcus aureus, and Pseudomonas aeruginosa, evaluating both planktonic and biofilm growth. Flavones had only very low-level antibacterial activity alone. They did not reduce biofilm formation, but did reduce production of the important P. aeruginosa inflammatory mediator and ciliotoxin pyocyanin. However, flavones exhibited synergy against P. aeruginosa in the presence of antibiotics or recombinant human lysozyme. They also enhanced the efficacy of antimicrobials secreted by cultured and primary human airway cells grown at air-liquid interface. This suggests that flavones may have anti-gram-negative potential as topical therapeutics when combined with antibiotics or in the context of innate antimicrobials secreted by the respiratory or other epithelia. This may have an additive effect when combined with T2R14-activated NO production. Additional studies are necessary to understand which flavone compounds or mixtures are the most efficacious.
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影响因子：
5
作者：
Babu, PBR;Chidekel, A;Shaffer, TH
通讯作者：
Shaffer, TH
影响因子：
3.1
作者：
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SHRYOCK, T
影响因子：
3.2
作者：
ESSAR, DW;EBERLY, L;CRAWFORD, IP
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CRAWFORD, IP
影响因子：
2.6
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Park, H