Partial expression of catecholaminergic traits in cholinergic chick ciliary ganglia: studies in vivo and in vitro.
Partial expression of catecholaminergic traits in cholinergic chick ciliary ganglia: studies in vivo and in vitro.
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胆碱能鸡睫状神经节儿茶酚胺能特征的部分表达：体内和体外研究。
DOI：
10.1016/0012-1606(85)90099-5
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发表时间：
1985
影响因子：
2.7
通讯作者：
G. Teitelman
中科院分区：
文献类型：
--
作者：
L. Iacovitti;T. Joh;V. Albert;D. H. Park;D. Reis;G. Teitelman
我们先前已经证明，在胚胎第8天，鸡睫状神经节(CG)的一些细胞含有儿茶酚胺(CA)酶酪氨酸羟化酶(TH)，但不含苯乙醇胺N-甲基转移酶(PNMT)；在培养中，基本上所有细胞都表达这两种酶。在目前的研究中，我们试图确定，首先，肾上腺素能特征在CGin活体中的表达在CG中是暂时的还是永久性的。为此，从E5到出生后第5天取出CGs，固定，并对CA酶：TH、L氨基酸脱羧酶和PNMT进行免疫细胞化学定位。在所有发育阶段，部分CG神经元表达TH免疫反应(TH-IR)，且均含有AADC-IR。然而，没有一个细胞被PNMT抗体染色，这表明这些细胞稳定地表达一些CA酶，但不是全部。其次，我们检测了培养的CG神经元是否表达其他CA标记。CG神经元不包含可检测到的TH酶活性水平，也不运输和储存外源供应的单胺。这些结果表明，在体外培养的CG神经元中存在肾上腺素能功能所必需的某些特征，但不是所有特征。第三，我们试图确定CA在CG神经元中的表达是否受培养条件的改变的影响。培养的CG神经元即使在50%HCM或20mMKCl存在下生长5天仍能继续表达TH-IR。最后，用生化方法检测胆碱能酶胆碱乙酰转移酶(CAT)在CG培养中的表达。无论姐妹培养的CG神经元中是否存在TH-IR，CAT活性在体外培养的5-17天间增加了5倍。这些数据表明，在培养中至少有一部分CG神经元同时表达TH和CAT。我们的结论是，CG的有丝分裂后神经元能够表达一些但不是所有CA表型的特征，同时保持胆碱能表达。这些发现表明：(1)肾上腺素能特性的全部出现是不协调的，可能受不同环境线索的调节；(2)副交感神经元可以同时表达肾上腺素能和胆碱能特性。
We have previously demonstrated that at embryonic Day (E) 8, some cells of the chick ciliary ganglion (CG) contain the catecholaminergic (CA) enzyme tyrosine hydroxylase (TH), but not phenylethanolamine-N-methyltransferase (PNMT); and that in culture essentially all cells express both enzymes. In the present study, we sought to determine, first, whether the expression of adrenergic traits in the CGin vivois transient or permanent in the CG. To do so, CGs were removed from E5 to postnatal Day 5, fixed, and processed for the immunocytochemical localization of the CA enzymes: TH,l-amino acid decarboxylase (AADC), and PNMT. At all stages examined, some CG neurons expressed TH immunoreactivity (TH-IR) and all contained AADC-IR. However, none stained with PNMT antibodies, indicating that these cells stably express some, but not all, of the CA enzymes. Second, we examined whether CG neurons in culture expressed other CA markers. CG neurons did not contain detectable levels of TH enzyme activity nor did they transport and store exogenously supplied monoamines. These results indicate that some but not all traits necessary for adrenergic function are present in CG neuronsin vitro. Third, we sought to establish whether CA expression in CG neurons is affected by modification in culture conditions. Cultures of CG neurons continued to express TH-IR even when grown in the presence of either 50% HCM or 20 mMKCl for 5 days. Finally, the expression of the cholinergic enzyme, choline acetyltransferase (CAT) was assessed in CG cultures by biochemical assay. CAT activity increased five-fold between 5 and 17 daysin vitro, irrespective of the presence of TH-IR in 100% of the CG neurons of sister cultures. These data suggest that at least a subpopulation of CG neurons express both TH and CAT in culture. We conclude that the postmitotic neurons of the CG are able to express some but not all of the traits characteristic of a CA phenotype while maintaining cholinergic expression. These findings suggest that (1) the appearance of the full complement of adrenergic properties is not coordinated and may be regulated by different environmental cues and (2) parasympathetic neurons can express both adrenergic and cholinergic traits simultaneously.
儿茶酚胺能前体转化为小鼠胚胎胰腺中的胰高血糖素 (A) 细胞。
DOI：
10.1073/pnas.78.8.5225
发表时间：
1981
影响因子：
11.1
作者：
Teitelman,G;Joh,TH;Reis,DJ
通讯作者：
Reis,DJ
体内神经递质可塑性的证据：胆碱能交感神经元特性的发育变化。
影响因子：
2.7
作者：
Landis,SC;Keefe,D
通讯作者：
Keefe,D
高钾浓度对细胞培养中睫状神经节神经元生长和发育的影响。
影响因子：
2.7
作者：
Nishi,R;Berg,DK
通讯作者：
Berg,DK