Rapid Multi-Hybridisation FISH Screening for Balanced Porcine Reciprocal Translocations Suggests a Much Higher Abnormality Rate Than Previously Appreciated.
Rapid Multi-Hybridisation FISH Screening for Balanced Porcine Reciprocal Translocations Suggests a Much Higher Abnormality Rate Than Previously Appreciated.
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DOI：
10.3390/cells10020250
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发表时间：
2021-01-28
期刊：
影响因子：
6
通讯作者：
Griffin DK
中科院分区：
文献类型：
--
作者：
O'Connor RE;Kiazim LG;Rathje CC;Jennings RL;Griffin DK
随着需求的增长，猪成为世界上肉类蛋白质的主要来源；然而，如果用于人工授精 (AI) 的公猪繁殖力低下（繁殖能力差），可能会造成重大经济损失和环境破坏。越来越多的证据表明，精液分析并不是诊断低生育率的可靠工具，产仔数和产仔率更适用。然而，一旦获得此类数据，任何受影响的公猪都将已经服役一段时间，并造成重大的财务和环境损失。相互易位（RT）是猪繁殖力低下的主要原因，据报道存在于 0.47% 的人工授精公猪中。然而，传统的标准核型分析依赖于动物特定的专业知识，并且不能检测更微妙（神秘）的易位。此前，我们报道了多重杂交荧光原位杂交（FISH）策略的开发；在这里，我们报告了它在 1641 AI 公猪中的使用情况。在 69 头公猪中总共鉴定出 15 种不同的 RT，其中还有 4 头动物是 XX/XY 嵌合体。因此，4.5% 的人存在染色体异常（4.2% 的人进行 RT），发生率为 0.88%。通过核型和 FISH 信息重新审视病例，我们重新分析了捕获的图像，询问仅通过核型分析是否可以检测到易位。结果表明，公猪的染色体易位可能被严重低估，从而凸显了在公猪进入育种计划之前通过这种方法进行预先筛选的必要性。
With demand rising, pigs are the world’s leading source of meat protein; however significant economic loss and environmental damage can be incurred if boars used for artificial insemination (AI) are hypoprolific (sub-fertile). Growing evidence suggests that semen analysis is an unreliable tool for diagnosing hypoprolificacy, with litter size and farrowing rate being more applicable. Once such data are available, however, any affected boar will have been in service for some time, with significant financial and environmental losses incurred. Reciprocal translocations (RTs) are the leading cause of porcine hypoprolificacy, reportedly present in 0.47% of AI boars. Traditional standard karyotyping, however, relies on animal specific expertise and does not detect more subtle (cryptic) translocations. Previously, we reported development of a multiple hybridisation fluorescence in situ hybridisation (FISH) strategy; here, we report on its use in 1641 AI boars. A total of 15 different RTs were identified in 69 boars, with four further animals XX/XY chimeric. Therefore, 4.5% had a chromosome abnormality (4.2% with an RT), a 0.88% incidence. Revisiting cases with both karyotype and FISH information, we reanalysed captured images, asking whether the translocation was detectable by karyotyping alone. The results suggest that chromosome translocations in boars may be significantly under-reported, thereby highlighting the need for pre-emptive screening by this method before a boar enters a breeding programme.
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影响因子：
1.7
作者：
Ducos, A.;Revay, T.;Iannuzzi, L.
通讯作者：
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作者：
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通讯作者：
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影响因子：
2.4
作者：
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通讯作者：
Griffin DK
影响因子：
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作者：
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通讯作者：
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