A serological survey of SARS-CoV-2 in cat in Wuhan.
A serological survey of SARS-CoV-2 in cat in Wuhan.
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DOI：
10.1080/22221751.2020.1817796
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发表时间：
2020-12
影响因子：
13.2
通讯作者：
Jin M
中科院分区：
文献类型：
--
作者：
Zhang Q;Zhang H;Gao J;Huang K;Yang Y;Hui X;He X;Li C;Gong W;Zhang Y;Zhao Y;Peng C;Gao X;Chen H;Zou Z;Shi ZL;Jin M
新冠肺炎是由SARS-CoV-2引起的一种新的呼吸道疾病，已构成全球性突发公共卫生事件。猫对SARS-CoV-2易感。然而，SARS-CoV-2在猫中的流行情况在很大程度上仍不清楚。采用血清学检测方法，对武汉市新冠肺炎暴发期间猫科动物感染SARS-CoV-2的情况进行了调查。武汉采集了一组猫的血清样本，其中102份是在新冠肺炎爆发后采集的，39份是在疫情爆发前采集的。经间接酶联免疫吸附试验(EL ISA)检测，15份SARS-CoV-2受体结合区(RBD)阳性。其中，11例SARS-CoV-2中和抗体滴度在1/20至1/1080之间。未发现SARS-CoV-2与猫传染性腹膜炎病毒(FIPV)的血清学交叉反应。此外，我们还连续监测了130天中每10天两只阳性猫的血清抗体动态。血清抗体在首次抽检后10d达到高峰，110d内降至检出限。本研究结果表明，新冠肺炎暴发期间，武汉地区猫科动物感染了SARS-CoV-2病毒，描述了猫科动物血清抗体的动态变化，为临床防治SARS提供了重要参考。
COVID-19 is a new respiratory illness caused by SARS-CoV-2, and has constituted a global public health emergency. Cat is susceptible to SARS-CoV-2. However, the prevalence of SARS-CoV-2 in cats remains largely unknown. Here, we investigated the infection of SARS-CoV-2 in cats during COVID-19 outbreak in Wuhan by serological detection methods. A cohort of serum samples were collected from cats in Wuhan, including 102 sampled after COVID-19 outbreak, and 39 prior to the outbreak. Fifteen sera collected after the outbreak were positive for the receptor binding domain (RBD) of SARS-CoV-2 by indirect enzyme linked immunosorbent assay (ELISA). Among them, 11 had SARS-CoV-2 neutralizing antibodies with a titer ranging from 1/20 to 1/1080. No serological cross-reactivity was detected between SARS-CoV-2 and type I or II feline infectious peritonitis virus (FIPV). In addition, we continuously monitored serum antibody dynamics of two positive cats every 10 days over 130 days. Their serum antibodies reached the peak at 10 days after first sampling, and declined to the limit of detection within 110 days. Our data demonstrated that SARS-CoV-2 has infected cats in Wuhan during the outbreak and described serum antibody dynamics in cats, providing an important reference for clinical treatment and prevention of COVID-19.
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影响因子：
4.3
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通讯作者：
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