Independent and additive effects of glutamic acid and methionine on yeast longevity.
Independent and additive effects of glutamic acid and methionine on yeast longevity.
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DOI：
10.1371/journal.pone.0079319
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Huang D
中科院分区：
文献类型：
--
作者：
Wu Z;Song L;Liu SQ;Huang D
葡萄糖限制可以延长酵母的时间和复制寿命，但关于氨基酸对酵母寿命的影响知之甚少，尽管有报道称一些氨基酸可以延缓啮齿动物的衰老。在这里，我们表明，氨基酸组成大大改变酵母的时间寿命。我们发现，非必需氨基酸（对酵母）蛋氨酸和谷氨酸对酵母的时间寿命延长有最显著的影响，限制蛋氨酸和/或增加谷氨酸导致寿命延长，这不是低乙酸产生和老化培养基酸化的结果。值得注意的是，低蛋氨酸，高谷氨酸和葡萄糖限制添加和独立地延长酵母寿命，这不能通过缓冲介质（pH 6.0）进一步延长。我们使用基因缺失的酵母的初步研究结果表明，谷氨酸添加，蛋氨酸和葡萄糖限制通过不同的机制提示酵母寿命。这项研究可能有助于填补酵母模型的空白，快速发展的观点认为营养平衡是延长寿命的关键因素。
It is established that glucose restriction extends yeast chronological and replicative lifespan, but little is known about the influence of amino acids on yeast lifespan, although some amino acids were reported to delay aging in rodents. Here we show that amino acid composition greatly alters yeast chronological lifespan. We found that non-essential amino acids (to yeast) methionine and glutamic acid had the most significant impact on yeast chronological lifespan extension, restriction of methionine and/or increase of glutamic acid led to longevity that was not the result of low acetic acid production and acidification in aging media. Remarkably, low methionine, high glutamic acid and glucose restriction additively and independently extended yeast lifespan, which could not be further extended by buffering the medium (pH 6.0). Our preliminary findings using yeasts with gene deletion demonstrate that glutamic acid addition, methionine and glucose restriction prompt yeast longevity through distinct mechanisms. This study may help to fill a gap in yeast model for the fast developing view that nutrient balance is a critical factor to extend lifespan.
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影响因子：
7.8
作者：
Alvers AL;Fishwick LK;Wood MS;Hu D;Chung HS;Dunn WA Jr;Aris JP
通讯作者：
Aris JP
DOI：
10.1126/science.1173635
发表时间：
2009-07-10
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Colman RJ;Anderson RM;Johnson SC;Kastman EK;Kosmatka KJ;Beasley TM;Allison DB;Cruzen C;Simmons HA;Kemnitz JW;Weindruch R
通讯作者：
Weindruch R
影响因子：
3.9
作者：
Goldberg, Alexander A.;Bourque, Simon D.;Titorenko, Vladimir I.
通讯作者：
Titorenko, Vladimir I.
影响因子：
4.4
作者：
Elshorbagy, Amany K.;Valdivia-Garcia, Maria;Perrone, Carmen E.
通讯作者：
Perrone, Carmen E.
DOI：
10.1083/jcb.200404002
发表时间：
2004-09-27
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fabrizio P;Battistella L;Vardavas R;Gattazzo C;Liou LL;Diaspro A;Dossen JW;Gralla EB;Longo VD
通讯作者：
Longo VD