Identification of a divergent O-acetyltransferase gene oac 1b from Shigella flexneri serotype 1b strains.
Identification of a divergent O-acetyltransferase gene oac 1b from Shigella flexneri serotype 1b strains.
复制标题
DOI：
10.1038/emi.2012.22
复制
发表时间：
2012-09
影响因子：
13.2
通讯作者：
Xu, Jianguo
中科院分区：
文献类型：
--
作者：
Sun, Qiangzheng;Lan, Ruiting;Wang, Yan;Wang, Jianping;Xia, Shengli;Wang, Yiting;Zhang, Jin;Yu, Deshan;Li, Zhenjun;Jing, Huaiqi;Xu, Jianguo
福氏志贺氏菌是发展中国家细菌性痢疾的主要病因。在已知的15种血清型中，有4种（1b、3a、3b和4b）含有6组表位，这是由于鼠李糖III在脂多糖的四糖结构上的O-2位置上有一个乙酰基连接。由噬菌体Sf6编码的o -乙酰转移酶介导乙酰化反应。我们发现血清型1b菌株的oac基因与血清型3a、3b和4b菌株的oac基因差异很大，在DNA水平上与oac有88%-89%的同源性，在蛋白水平上有85%的同源性，因此我们将其称为oac1b。考虑到血清型1-5的flexneri菌株具有最近的共同祖先，不同的oac1b更有可能是从flexneri外获得的，而不是与flexneri内其他血清型（3a， 3b和4b）中的oac基因发生快速分化。克隆的oac1b基因具有与oac相同的乙酰化功能。对oac1b侧翼基因组区域的分析表明，它存在于染色体上的一个噬菌体中，其组织结构与噬菌体Sf6不同。此外，噬菌体转化实验表明，用Sf6感染1a血清型菌株不能产生1b血清型。我们得出结论，oac1b由非sf6噬菌体携带，并且只存在于1b血清型中。
Shigella flexneri is a leading cause of bacterial dysentery in developing countries. Among the 15 known serotypes, four (1b, 3a, 3b and 4b) contain a group 6 epitope due to an acetyl group connected to the O-2 position of rhamnose III on the tetrasaccharide structure of the lipopolysaccharide. O-acetyltransferase encoded by a bacteriophage, Sf6, mediates the acetylation reaction. We found that the oac gene in serotype 1b strains was very different from that in serotypes 3a, 3b and 4b strains and is herein after referred to as oac1b which shares with oac 88%–89% identity at the DNA level and 85% identity at the protein level. Considering that S. flexneri strains of serotypes 1–5 share a recent common ancestry, the divergent oac1b is more likely to have been obtained from outside S. flexneri than to have undergone rapid divergence from the oac gene in the other serotypes (3a, 3b and 4b) within S. flexneri. The cloned oac1b gene was found to perform the same acetylation function as oac. Analysis of the genomic regions flanking oac1b showed that it was present in a prophage on the chromosome and the organizational structure is different from that of phage Sf6. Additionally, phage conversion assay showed that serotype 1b cannot be generated by infecting serotype 1a strains with Sf6. We conclude that oac1b was carried by a non-Sf6 phage and is uniquely present in serotype 1b.
登录
查看更多内容
DOI：
10.1099/13500872-145-7-1649
发表时间：
1999-07-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Chua, JEH;Manning, PA;Morona, R
通讯作者：
Morona, R
影响因子：
5.4
作者：
LINDBERG, AA;WOLLIN, R;WOHLHIETER, JA
通讯作者：
WOHLHIETER, JA
影响因子：
5.6
作者：
Casjens, S;Winn-Stapley, DA;Clark, AJ
通讯作者：
Clark, AJ
影响因子：
11.8
作者：
Bardhan P;Faruque AS;Naheed A;Sack DA
通讯作者：
Sack DA
影响因子：
15.8
作者：
von Seidlein L;Kim DR;Ali M;Lee H;Wang X;Thiem VD;Canh DG;Chaicumpa W;Agtini MD;Hossain A;Bhutta ZA;Mason C;Sethabutr O;Talukder K;Nair GB;Deen JL;Kotloff K;Clemens J
通讯作者：
Clemens J