Gas-phase bioproduction of a high-value-added monoterpenoid (E)-geranic acid by metabolically engineered Acinetobacter sp. Tol 5
Gas-phase bioproduction of a high-value-added monoterpenoid (E)-geranic acid by metabolically engineered Acinetobacter sp. Tol 5
复制标题
通过代谢工程不动杆菌气相生物生产高附加值单萜 (E)-香叶酸。
DOI：
10.1039/c9gc03478a
复制
发表时间：
2020
期刊：
影响因子：
9.8
通讯作者：
Hori Katsutoshi
中科院分区：
文献类型：
--
作者：
Usami Atsushi;Ishikawa Masahito;Hori Katsutoshi
采用固定化生物催化剂并在气相中进行化学反应的气相生物生产作为一种绿色工艺引起了研究人员的关注。然而，由于缺乏合适的细胞固定化方法，全细胞催化剂在气相生物生产中的应用存在困难。不动杆菌属。TOL 5是一种独特的细菌，它具有极强的粘性，可以通过粘附性细菌纤维蛋白Ataa很容易地固定在各种材料表面。在这项研究中，我们展示了利用固定化Tol 5转化细胞从香叶醇中气相生物合成(E)-香叶酸(GA)的方法，其中引入了一个参与(E)-GA合成途径的基因。(E)-GA液相生物合成的时间进程分析揭示了TOL5参与(E)-GA降解的内在代谢。通过破坏编码(E)-GA降解关键酶的FADD4基因，我们成功地产生了一株积累(E)-GA的菌株TOL5ΔFADD4(PGeA)。这种突变菌株的固定化细胞在聚氨酯载体上，能够在间歇气相反应中被动供应气态香叶醇来生产(E)-GA。产生的(E)-GA的大部分被吸附在聚氨酯载体上，但很容易被提取到乙醇中，乙醇是一种安全的溶剂，不会对环境造成影响。这是第一个气相生物生产复杂和高附加值化合物的例子。TOL 5是一种非常有前途的气相生物生产平台。
Gas-phase bioproduction, in which immobilized biocatalysts are employed and chemical reactions are performed in a gas phase, has attracted researchers’ attention as a green process. However, there is difficulty in the employment of whole cell catalysts for gas-phase bioproduction due to the lack of a suitable cell immobilization method. Acinetobacter sp. Tol 5 is a unique bacterium, which is remarkably sticky and can be easily immobilized onto various material surfaces through the adhesive bacterionanofiber protein AtaA. In this study, we demonstrate the gas-phase bioproduction of (E)-geranic acid (GA), a high-value-added monoterpenoid, from geraniol using immobilized Tol 5 transformant cells, into which a gene involved in a (E)-GA synthetic pathway was introduced. Time course analysis of the liquid-phase bioproduction of (E)-GA revealed the inherent metabolism of Tol 5 involved in the degradation of (E)-GA. By disrupting the fadD4-ortholog gene, which encodes a key enzyme of the (E)-GA degradation, we successfully generated a (E)-GA-accumulating strain, Tol 5 ΔfadD4 (pGeoA). The immobilized cells of this mutant strain on a polyurethane support enabled the production of (E)-GA with a passive supply of gaseous geraniol in a batch gas-phase reaction. A major fraction of the (E)-GA, which was produced, was adsorbed onto the polyurethane support but easily extracted into ethanol, a safe solvent without environmental impact. This is the first example of gas-phase bioproduction of a complex and high-value-added compound. Tol 5 is a highly promising platform for gas-phase bioproduction.
登录
查看更多内容
难溶于水的酮的对映选择性还原：水性缓冲系统的连续处理和回收
影响因子：
9.8
作者：
Susanne Leuchs;Shukrallah Na'amnieh;Lasse Greiner
通讯作者：
Lasse Greiner
影响因子：
4.7
作者：
Stefan Tippmann;Yun Chen;V. Siewers;J. Nielsen
通讯作者：
Stefan Tippmann;Yun Chen;V. Siewers;J. Nielsen
更快更好的生物催化过程：在有机合成中实施生物催化的新工具
DOI：
--
发表时间：
2002
期刊：
影响因子：
--
作者：
G. Lye;P. Dalby;J. Woodley
通讯作者：
J. Woodley
影响因子：
6.1
作者：
Chen, Liang;Capone, Dimitra L.;Jeffery, David W.
通讯作者：
Jeffery, David W.