Ketamine Anesthesia on Neurocyte in Fetal Rat via cAMP and NO/cGMP Pathway
Ketamine Anesthesia on Neurocyte in Fetal Rat via cAMP and NO/cGMP Pathway
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氯胺酮通过 cAMP 和 NO/cGMP 途径麻醉胎鼠神经细胞
DOI：
10.29261/pakvetj/2018.113
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发表时间：
2019-04
影响因子：
2.3
通讯作者：
Li Gao
中科院分区：
文献类型：
--
作者：
Yu Chen;Wenhan Liu;Mo Liu;Jinghua Zhao;Xinran Li;Tian Chang;Qi Wang;Xintong Zhang;Daiyue Chang;Li Gao
收稿日期: 修订日期: 已接受日期: 在线发布日期: May 13, 2018 October 08, 2018 October 14, 2018 December 21, 2018 婴儿期氯胺酮麻醉对神经系统发育有影响。本研究旨在证明氯胺酮通过 cAMP 和 NO-cGMP 途径对神经细胞的影响。 Wistar胎鼠皮质神经细胞分为四组。各组给予不同剂量,低剂量组0.7 μg/mL,麻醉组分别在0、5、10、15、20、25、30、45、60、90、120 min时1 μg/mL、3 μg/mL、5 μg/mL。采用ELISA法检测cGMP和cAMP含量，采用分光光度法检测NOS活性和NO含量。对离体神经元施加不同浓度的氯胺酮后，cAMP和cGMP水平先降低后升高，25 min时含量达到底部，然后升高。 30 min内,NO和NOS含量分别先下降至最低值,然后又上升。在120分钟内，它们的值均低于0分钟。氯胺酮的麻醉作用可能是通过增加cAMP和抑制NO-cGMP途径来实现的。 ©2018 PVJ。版权所有
Received: Revised: Accepted: Published online: May 13, 2018 October 08, 2018 October 14, 2018 December 21, 2018 Ketamine anesthesia during infancy has an impact on development of nervous system. This study is to demonstrate effects of ketamine on neuronal cells via cAMP and NO-cGMP pathway. The cortex neurocyte of Wistar fetal rat were divided into four groups. Every group was given different doses, low dose group was 0.7 μg/mL, anesthesia groups were 1 μg/mL, 3 μg/mL, 5 μg/mL at 0, 5, 10, 15, 20, 25, 30, 45, 60, 90 and 120 min, respectively. The content of cGMP and cAMP were detected by ELISA, and the activity of NOS and content of NO were detected by spectrophotometric method. After different concentrations of ketamine were applied to isolated neurons, cAMP and cGMP levels were lowered first and then increased, the content reached the bottom at 25 min and then increased. In the 30 min, the content of NO and NOS decreased to the lowest value and then went up, respectively. In the 120 min, their values were both lower than 0 min. Ketamine probably expressed its anesthesia effect with the increasement of cAMP and inhibition of NO-cGMP pathway. ©2018 PVJ. All rights reserved
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影响因子：
2.7
作者：
Zhuanghui Zhu;Shuanglian Yi;Z. Shan;Hongwei Guo;Shaofan Ke
通讯作者：
Zhuanghui Zhu;Shuanglian Yi;Z. Shan;Hongwei Guo;Shaofan Ke
影响因子：
3.7
作者：
T. Yagami;K. Takase;Yasuhiro Yamamoto;K. Ueda;N. Takasu;N. Okamura;T. Sakaeda;M. Fujimoto
通讯作者：
T. Yagami;K. Takase;Yasuhiro Yamamoto;K. Ueda;N. Takasu;N. Okamura;T. Sakaeda;M. Fujimoto
DOI：
--
发表时间：
2008
期刊：
Chinese Journal of Animal and Veterinary Sciences
影响因子：
--
作者：
Zhang Jian-tao
通讯作者：
Zhang Jian-tao
影响因子：
9.8
作者：
Galley, HF;Le Cras, AE;Webster, NR
通讯作者：
Webster, NR
影响因子：
8.8
作者：
Y. Vulliemoz;Hong Shen;L. Virág
通讯作者：
Y. Vulliemoz;Hong Shen;L. Virág