The diversity of bacteria isolated from antarctic freshwater reservoirs possessing the ability to produce polyhydroxyalkanoates.
The diversity of bacteria isolated from antarctic freshwater reservoirs possessing the ability to produce polyhydroxyalkanoates.
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DOI：
10.1007/s00284-014-0629-1
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发表时间：
2014-11
影响因子：
2.6
通讯作者：
Zdanowski, Marek
中科院分区：
文献类型：
--
作者：
Ciesielski, Slawomir;Gorniak, Dorota;Mozejko, Justyna;Swiatecki, Aleksander;Grzesiak, Jakub;Zdanowski, Marek
通过依赖培养的方法检查了南极洲生态冰川前陆淡水水库中产生聚羟基脂肪酸酯的细菌的多样性。通过 16S rRNA 基因测序对分离的菌株进行系统发育分析，并将其分类为 Alpha、Beta 或 Gammaproteobacteria 类的成员。采用聚合酶链式反应检测PHA合酶基因。潜在的聚羟基脂肪酸酯（PHA）生产者主要属于假单胞菌属和詹蒂诺杆菌属。从所有五个采样点均分离出 PHA，这表明 PHA 合成是先锋站点的常见细菌特征。所有假单胞菌菌株都具有合成中链长度 PHA 的遗传潜力，而一些分离的詹提诺杆菌菌株可能产生短链长度 PHA 或中链长度 PHA。这是第一份揭示詹蒂诺杆菌属物种可能具有生产中链长度 PHA 的潜力的报告。
The diversity of polyhydroxyalkanoates-producing bacteria in freshwater reservoirs in the Ecology Glacier foreland, Antarctica, was examined by a cultivation-dependent method. Isolated strains were analyzed phylogenetically by 16S rRNA gene sequencing, and classified as members of Alpha-, Beta-, or Gammaproteobacteria classes. Polymerase chain reaction was used to detect PHA synthase genes. Potential polyhydroxyalkanoates (PHAs) producers belonging mainly to Pseudomonas sp., and Janthinobacterium sp. were isolated from all five sampling sites, suggesting that PHA synthesis is a common bacterial feature at pioneer sites. All Pseudomonas strains had the genetic potential to synthesize medium-chain-length PHAs, whereas some isolated Janthinobacterium strains might produce short-chain-length PHAs or medium-chain-length PHAs. It is the first report revealing that Janthinobacterium species could have the potential to produce medium-chain-length PHAs.
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影响因子：
11.4
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1007/s10295-010-0698-5
发表时间：
2010-05-01
影响因子：
3.4
作者：
Ciesielski, Slawomir;Mozejko, Justyna;Przybylek, Grzegorz
通讯作者：
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