Network oscillations drive correlated spiking of ON and OFF ganglion cells in the rd1 mouse model of retinal degeneration.
Network oscillations drive correlated spiking of ON and OFF ganglion cells in the rd1 mouse model of retinal degeneration.
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DOI：
10.1371/journal.pone.0086253
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Detwiler PB
中科院分区：
文献类型：
--
作者：
Margolis DJ;Gartland AJ;Singer JH;Detwiler PB
在光感受器变性后，rd-1/rd-1小鼠的视网膜神经节细胞（RGCs）接受有节奏的突触输入，引发约10hz的动作电位爆发。为了表征这种活动的性质，我们利用双光子成像技术对RGCs进行配对记录和形态学分类，分别为ON α、OFF α和非α RGCs。所鉴定的细胞类型表现出节律性的尖峰活动。从成对的RGCs同时记录的脉冲序列相互关联显示，α RGCs之间的活动相关性比α和非α细胞对之间的活动相关性更强。相同类型的α RGC对（即两个ON或两个OFF细胞）的动作电位爆发是同相的，而不同类型的α细胞（即一个ON和一个OFF的RGC）的动作电位爆发是180度的。这一结果与RGC活动是由一个输入驱动的，该输入为ON细胞提供相关的兴奋，并对OFF细胞提供抑制。A2 amacrine细胞作为一种候选细胞机制进行了研究，发现在快速突触传递拮抗剂存在的情况下，膜电压和电流表现出10hz的振荡，并被河豚毒素消除。结果支持以下结论：RGC节律性活动起源于包括A2s在内的电偶联细胞的突触前网络，其机制依赖于Na+通道。网络活动驱动开和关锥形双极细胞的相位振荡，在视网膜区域的RGC尖峰活动中产生类似的频率波动，该区域随着细胞振荡器空间位置的变化而迁移。
Following photoreceptor degeneration, ON and OFF retinal ganglion cells (RGCs) in the rd-1/rd-1 mouse receive rhythmic synaptic input that elicits bursts of action potentials at ∼10 Hz. To characterize the properties of this activity, RGCs were targeted for paired recording and morphological classification as either ON alpha, OFF alpha or non-alpha RGCs using two-photon imaging. Identified cell types exhibited rhythmic spike activity. Cross-correlation of spike trains recorded simultaneously from pairs of RGCs revealed that activity was correlated more strongly between alpha RGCs than between alpha and non-alpha cell pairs. Bursts of action potentials in alpha RGC pairs of the same type, i.e. two ON or two OFF cells, were in phase, while bursts in dissimilar alpha cell types, i.e. an ON and an OFF RGC, were 180 degrees out of phase. This result is consistent with RGC activity being driven by an input that provides correlated excitation to ON cells and inhibition to OFF cells. A2 amacrine cells were investigated as a candidate cellular mechanism and found to display 10 Hz oscillations in membrane voltage and current that persisted in the presence of antagonists of fast synaptic transmission and were eliminated by tetrodotoxin. Results support the conclusion that the rhythmic RGC activity originates in a presynaptic network of electrically coupled cells including A2s via a Na+-channel dependent mechanism. Network activity drives out of phase oscillations in ON and OFF cone bipolar cells, entraining similar frequency fluctuations in RGC spike activity over an area of retina that migrates with changes in the spatial locus of the cellular oscillator.
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影响因子：
5.3
作者：
Margolis, David J.;Detwiler, Peter B.
通讯作者：
Detwiler, Peter B.
DOI：
10.1523/jneurosci.5694-09.2010
发表时间：
2010-05-26
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Margolis DJ;Gartland AJ;Euler T;Detwiler PB
通讯作者：
Detwiler PB
影响因子：
1.9
作者：
Demb, Jonathan B.;Singer, Joshua H.
通讯作者：
Singer, Joshua H.
影响因子：
4.5
作者：
Euler, Thomas;Hausselt, Susanne E.;Margolis, David J.;Breuninger, Tobias;Castell, Xavier;Detwiler, Peter B.;Denk, Winfried
通讯作者：
Denk, Winfried
影响因子：
25
作者：
Murphy, Gabe J.;Rieke, Fred
通讯作者：
Rieke, Fred