Eyecup scope--optical recordings of light stimulus-evoked fluorescence signals in the retina.
Eyecup scope--optical recordings of light stimulus-evoked fluorescence signals in the retina.
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DOI：
10.1007/s00424-008-0603-5
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发表时间：
2009-04
影响因子：
4.5
通讯作者：
Denk, Winfried
中科院分区：
文献类型：
--
作者：
Euler, Thomas;Hausselt, Susanne E.;Margolis, David J.;Breuninger, Tobias;Castell, Xavier;Detwiler, Peter B.;Denk, Winfried
关键词：
树突信号在处理视网膜视觉信息中发挥着重要作用。为了在太小而无法进行电记录的神经突中研究它们，我们开发了一种将多光子（MP）显微镜与透过物镜的高分辨率视觉刺激器相结合的仪器。设计了一种正置显微镜，使用物镜进行 MP 成像并将视觉刺激传递到功能完整的视网膜外植体或眼杯制备物。该刺激器由一个微型硅基液晶显示器组成，该显示器耦合到红外激发激光扫描显微镜的光路中。一对定制的二向色滤光片允许来自激发激光的光和来自刺激器的三个光谱带（“颜色”）到达视网膜，留下两个中间波段用于荧光成像。特殊的光学器件允许刺激器焦点相对于成像焦点的位移。在不同类型的哺乳动物视网膜神经元的树突中记录了响应视觉刺激的钙指示剂荧光的空间分辨变化。
Dendritic signals play an essential role in processing visual information in the retina. To study them in neurites too small for electrical recording, we developed an instrument that combines a multi-photon (MP) microscope with a through-the-objective high-resolution visual stimulator. An upright microscope was designed that uses the objective lens for both MP imaging and delivery of visual stimuli to functionally intact retinal explants or eyecup preparations. The stimulator consists of a miniature liquid-crystal-on-silicon display coupled into the optical path of an infrared-excitation laser-scanning microscope. A pair of custom-made dichroic filters allows light from the excitation laser and three spectral bands (‘colors’) from the stimulator to reach the retina, leaving two intermediate bands for fluorescence imaging. Special optics allow displacement of the stimulator focus relative to the imaging focus. Spatially resolved changes in calcium-indicator fluorescence in response to visual stimuli were recorded in dendrites of different types of mammalian retinal neurons.
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影响因子：
64.8
作者：
Euler, T;Detwiler, PB;Denk, W
通讯作者：
Denk, W
影响因子：
5.5
作者：
Gray-Keller, M;Denk, W;Detwiler, PB
通讯作者：
Detwiler, PB
影响因子：
--
作者：
ENOCH, JM
通讯作者：
ENOCH, JM
影响因子：
5.5
作者：
BAYLOR, DA;FETTIPLACE, R
通讯作者：
FETTIPLACE, R
影响因子：
1.8
作者：
LAMB, TD
通讯作者：
LAMB, TD