Stimulation of glucose production by activin-A in isolated rat hepatocytes.
Stimulation of glucose production by activin-A in isolated rat hepatocytes.
复制标题
激活素 A 在离体大鼠肝细胞中刺激葡萄糖产生。
作者：
T. Mine;I. Kojima;E. Ogata
本文研究了激活素-A对大鼠肝细胞糖原分解的影响。激活素-A以剂量依赖的方式刺激肝细胞葡萄糖输出。激活素-A的糖原分解作用在10(-9)M时达到最大，约为胰升糖素作用的50%。当激活素-A和5×10(-9)M的胰升糖素同时加入时，两者的作用是相加的。相反，当加入10(-9)M激活素-A和10(-8)M血管紧张素-II时，没有可加性。激活素-A在所测试的任何剂量下均不增加cAMP，但可引起胞浆内游离钙浓度的迅速增加。激活素-A即使在1微米的细胞外钙离子存在的情况下也会增加胞浆内游离钙浓度，这表明激活素-A引起细胞内钙池的钙释放(S)。激活素-A影响的内部钙池似乎与血管紧张素-II或血管加压素影响的相同。当[~3H]肌醇标记的肝细胞与激活素-A孵育时，肌醇三磷酸部分的放射性迅速增加。这些结果表明激活素-A作用于大鼠肝细胞，并通过激活钙信使系统刺激糖原分解。
The effect of activin-A on glycogenolysis was studied in isolated rat hepatocytes. Activin-A stimulated glucose output in hepatocytes in a dose-dependent manner. The maximal effect of the glycogenolytic action of activin-A, which was about 50% of the glucagon action, was obtained at 10(-9) M. When 10(-9) M activin-A and 5 x 10(-9) M glucagon were added simultaneously, the actions of these two agents were additive. In contrast, there was no additivity when 10(-9) M activin-A and 10(-8) M angiotensin-II were added. Activin-A did not increase cAMP at any doses tested, but induced a rapid increase in cytoplasmic free calcium concentration. Activin-A increased the cytoplasmic free calcium concentration even in the presence of 1 microM extracellular calcium, suggesting that activin-A caused calcium release from an intracellular calcium pool(s). The internal calcium pool affected by activin-A appeared to be the same as that affected by either angiotensin-II or vasopressin. When [3H] inositol-labeled hepatocytes were incubated with activin-A, radioactivity in the inositol trisphosphate fraction was rapidly increased. These results indicate that activin-A acts on rat hepatocytes and stimulates glycogenolysis by activating the calcium messenger system.
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GH3 细胞中分泌激活剂诱导的胞质离子钙的变化。
DOI：
10.1152/ajpendo.1984.246.2.e198
发表时间：
1984
期刊：
The American journal of physiology
影响因子：
--
作者：
Snowdowne,KW;Borle,AB
通讯作者：
Borle,AB
DOI：
10.1016/s0006-291x(88)80043-3
发表时间：
1988-11-30
影响因子：
3.1
作者：
GANAPATHI, MK;MAY, LT;KUSHNER, I
通讯作者：
KUSHNER, I
钙和磷酸肌醇在某些激素和神经递质的作用中的作用。
DOI：
10.1172/jci111886
发表时间：
1985
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Exton,JH
通讯作者：
Exton,JH
DOI：
10.1016/0167-4889(84)90071-5
发表时间：
1984-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
CORVERA, S;HUERTABAHENA, J;GARCIASAINZ, JA
通讯作者：
GARCIASAINZ, JA