Chloroplast translational regulation uncovers nonessential photosynthesis genes as key players in plant cold acclimation.
Chloroplast translational regulation uncovers nonessential photosynthesis genes as key players in plant cold acclimation.
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DOI：
10.1093/plcell/koac056
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发表时间：
2022-04-26
期刊：
影响因子：
--
通讯作者：
Zoschke R
中科院分区：
文献类型：
--
作者：
Gao Y;Thiele W;Saleh O;Scossa F;Arabi F;Zhang H;Sampathkumar A;Kühn K;Fernie A;Bock R;Schöttler MA;Zoschke R
植物进化出有效的多方面适应策略来应对低温。叶绿体对温度刺激作出反应并参与温度感知和适应。然而，人们对叶绿体基因及其表达在植物耐冷性中的作用知之甚少。在这里，我们通过检查叶绿体基因组拷贝数、转录物积累和翻译、光合作用、细胞生理学和代谢的反应，系统地研究了暴露在低温下 2 天的烟草幼苗的冷驯化。我们对叶绿体基因表达的时间分辨全基因组研究揭示了在起始和延伸水平上存在大量冷诱导的翻译调节，而转录本水平上几乎没有变化。这些冷触发的叶绿体翻译动力学与之前描述的强光诱导效应有很大不同。对冷刺激显着响应的基因组分析表明，光合蛋白复合物的非必需质体编码亚基是植物冷驯化中的新参与者。通过反向遗传学对这些冷响应叶绿体基因之一的功能表征表明，编码的蛋白质，即小细胞色素 b6f 复合体亚基 PetL，对光合作用冷驯化至关重要。总之，我们的结果揭示了叶绿体翻译调控在植物冷驯化中的一个重要的、以前未被充分认识的作用。冷暴露诱导质体编码基因的翻译调节，并揭示了一个小的非必需细胞色素 b6f 复合物亚基在寒冷适应中的关键作用。
Plants evolved efficient multifaceted acclimation strategies to cope with low temperatures. Chloroplasts respond to temperature stimuli and participate in temperature sensing and acclimation. However, very little is known about the involvement of chloroplast genes and their expression in plant chilling tolerance. Here we systematically investigated cold acclimation in tobacco seedlings over 2 days of exposure to low temperatures by examining responses in chloroplast genome copy number, transcript accumulation and translation, photosynthesis, cell physiology, and metabolism. Our time-resolved genome-wide investigation of chloroplast gene expression revealed substantial cold-induced translational regulation at both the initiation and elongation levels, in the virtual absence of changes at the transcript level. These cold-triggered dynamics in chloroplast translation are widely distinct from previously described high light-induced effects. Analysis of the gene set responding significantly to the cold stimulus suggested nonessential plastid-encoded subunits of photosynthetic protein complexes as novel players in plant cold acclimation. Functional characterization of one of these cold-responsive chloroplast genes by reverse genetics demonstrated that the encoded protein, the small cytochrome b6f complex subunit PetL, crucially contributes to photosynthetic cold acclimation. Together, our results uncover an important, previously underappreciated role of chloroplast translational regulation in plant cold acclimation. Cold exposure induces translational regulation of plastid-encoded genes and reveals a critical role of a small nonessential cytochrome b6f complex subunit in chilling acclimation.
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DOI：
10.1073/pnas.0406069101
发表时间：
2004-10-19
影响因子：
11.1
作者：
Cook, D;Fowler, S;Thomashow, MF
通讯作者：
Thomashow, MF
影响因子：
3.7
作者：
Grennan, Aleel K.;Ort, Donald R.
通讯作者：
Ort, Donald R.
影响因子：
2.9
作者：
Castelletti, S;Morosinotto, T;Croce, R
通讯作者：
Croce, R
影响因子：
5.8
作者：
Garcia-Molina, Antoni;Kleine, Tatiana;Leister, Dario
通讯作者：
Leister, Dario
DOI：
10.1007/s00249-008-0260-9
发表时间：
2008-09-01
影响因子：
2
作者：
Bogdanovic, J.;Mojovic, M.;Spasojevic, I.
通讯作者：
Spasojevic, I.