Identification of ultraviolet B radiation‑induced microRNAs in normal human dermal papilla cells.
Identification of ultraviolet B radiation‑induced microRNAs in normal human dermal papilla cells.
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DOI：
10.3892/mmr.2014.2418
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发表时间：
2014-10
影响因子：
3.4
通讯作者：
Bae S
中科院分区：
文献类型：
--
作者：
Cha HJ;Kim OY;Lee GT;Lee KS;Lee JH;Park IC;Lee SJ;Kim YR;Ahn KJ;An IS;An S;Bae S
紫外线（UV）辐射通过直接损伤DNA和间接产生活性氧（ROS）来损害细胞内功能，从而诱导细胞周期停滞和凋亡。紫外线辐射还可以改变基因表达谱，包括mRNA和microRNA（miRNA）的基因表达谱。紫外线辐射对细胞功能和基因表达的影响在人类皮肤细胞如角质形成细胞、黑素细胞和真皮成纤维细胞中已被广泛记录，但它对其他类型的皮肤细胞如毛乳头细胞的影响仍然未知，毛乳头细胞在诱导毛囊生长中至关重要。在本研究中，紫外线辐射对正常人毛乳头细胞（nHDPs）的生理变化和基于miRNA的表达谱的影响进行了研究。UVB辐射剂量≥50 mJ/cm 2时，细胞毒性和细胞凋亡呈剂量依赖性。此外，在UVB照射的nHDPs中表现出ROS的产生。此外，使用miRNA微阵列分析，结果表明，42个miRNA在UVB照射的nHDPs的表达谱显着改变相比，在对照组（35上调和7下调）。通过基因本体分析对差异表达的miRNAs的生物学功能进行了研究，以确定其推定的靶mRNA，并证明其参与细胞存活和死亡相关的功能。总之，本研究的结果提供了证据表明，基于miRNA的细胞机制可能参与UVB诱导的nHDPs细胞反应。
Ultraviolet (UV) radiation impairs intracellular functions by directly damaging DNA and by indirectly generating reactive oxygen species (ROS), which induce cell cycle arrest and apoptosis. UV radiation can also alter gene expression profiles, including those of mRNA and microRNA (miRNA). The effects of UV radiation on cellular functions and gene expression have been widely documented in human skin cells such as keratinocytes, melanocytes and dermal fibroblasts, but the effect it has on other types of skin cell such as dermal papilla cells, which are crucial in the induction of hair follicle growth, remains unknown. In the current study, the effect of UV radiation on physiological changes and miRNA-based expression profiles in normal human dermal papilla cells (nHDPs) was investigated. UVB radiation of ≥50 mJ/cm2 displayed high cytotoxicity and apoptosis in a dose-dependent manner. In addition, ROS generation was exhibited in UVB-irradiated nHDPs. Furthermore, using miRNA microarray analysis, it was demonstrated that the expression profiles of 42 miRNAs in UVB-irradiated nHDPs were significantly altered compared with those in the controls (35 upregulated and 7 downregulated). The biological functions of the differentially expressed miRNAs were studied with gene ontology analysis to identify their putative target mRNAs, and were demonstrated to be involved in cell survival- and death-related functions. Overall, the results of the present study provide evidence that miRNA-based cellular mechanisms may be involved in the UVB-induced cellular response in nHDPs.
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DOI：
10.1111/j.1600-0781.2010.00522.x
发表时间：
2010-08-01
影响因子：
2.6
作者：
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通讯作者：
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影响因子：
6.5
作者：
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通讯作者：
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影响因子：
3.4
作者：
Trüeb, RM
通讯作者：
Trüeb, RM
影响因子：
13.8
作者：
Wan, Guohui;Mathur, Rohit;Hu, Xiaoxiao;Zhang, Xinna;Lu, Xiongbin
通讯作者：
Lu, Xiongbin
影响因子：
5.2
作者：
Yamauchi, Takahiro;Adachi, Seiji;Moriwaki, Hisataka
通讯作者：
Moriwaki, Hisataka