Resonance Raman spectroscopy of the cytochrome c oxidase from Paracoccus denitrificans.
Resonance Raman spectroscopy of the cytochrome c oxidase from Paracoccus denitrificans.
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脱氮副球菌细胞色素 c 氧化酶的共振拉曼光谱。
DOI：
10.1021/bi00078a016
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发表时间：
1993
期刊：
影响因子：
2.9
通讯作者：
Copeland,RA
中科院分区：
文献类型：
--
作者：
Lynch,SR;Carter,RH;Copeland,RA
MATERIALS AND METHODSBovine cardiac mitochondria were isolated, and the cytochrome c oxidase from them was purified by the method of Hartzell and Beinert (1974). Submitochondrial particles were prepared as previously described (Ragan et al., 1987). Spheroplasts were prepared from whole cells of Paracoccus denitrificans by lysozyme/DNase treatment, as described by Ludwig and Schatz (1980). The enzyme from Paracoccus denitrificans was purified by a combination of ion-exchange chromatography on Q Sepharose FF and lysine affinity chromatography (Felsh et al., 1992). A detailed description of the purification method for this and otherprokaryotic oxidases will be presented separately (Horvath et al., un-published results). This purification method yields a highly active enzyme that retains all three of its subunits, in contrast to the earlier purification methods that yielded a two-subunit enzyme (Ludwig & Schatz, 1980). The purified enzymes were flash-frozen in a dry ice/ethanol bath and stored at-80 C until use. The fully reduced and cyanide-inhibited mixed-valence forms of these enzymes were prepared in low ionic strength buffer [50 mM 4-(2-hydrox-
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影响因子：
2.9
作者：
M. Lauraeus;Mårten Wikström;C. Varotsis;M. Tecklenburg;Gerald T. Babcock
通讯作者：
M. Lauraeus;Mårten Wikström;C. Varotsis;M. Tecklenburg;Gerald T. Babcock
可通过 EPR 光谱检测细胞色素 C 氧化酶的成分。
DOI：
10.1016/0005-2728(74)90178-9
发表时间：
1974
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
C. Hartzell;Helmut Beinert
通讯作者：
Helmut Beinert
通过赖氨酸亲和层析纯化细胞色素c氧化酶。
影响因子：
1.6
作者：
J. Felsch;S. Kotake;R. Copeland
通讯作者：
R. Copeland
DOI：
--
发表时间：
1979
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
M. Erecínska;D. Wilson;J. Blasie
通讯作者：
J. Blasie
aa3 型细胞色素氧化酶的铁-组氨酸拉伸拉曼线
影响因子：
15
作者：
T. Ogura;K. Hon;T. Oshima;S. Yoshikawa;T. Kitagawa
通讯作者：
T. Kitagawa