Multifunctional oxidosqualene cyclases and cytochrome P450 involved in the biosynthesis of apple fruit triterpenic acids.
Multifunctional oxidosqualene cyclases and cytochrome P450 involved in the biosynthesis of apple fruit triterpenic acids.
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DOI：
10.1111/nph.13996
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发表时间：
2016-09
期刊：
影响因子：
--
通讯作者：
Laing WA
中科院分区：
文献类型：
--
作者：
Andre CM;Legay S;Deleruelle A;Nieuwenhuizen N;Punter M;Brendolise C;Cooney JM;Lateur M;Hausman JF;Larondelle Y;Laing WA
苹果（Malus × domestica）在其果实角皮中积累了具有生物活性的熊烷型、齐墩烷型和狼烷型三萜，但它们的生物合成途径尚不清楚。我们使用基于同源性的方法鉴定和功能表征了两种新的氧化角鲨烯环化酶（MdOSC4和MdOSC5）和一种细胞色素P450 （CYP716A175）。在20个苹果品种中进一步研究了这些酶和先前描述的氧化角鲨烯环化酶的基因表达模式，并对比了三萜谱。MdOSC4编码一种多功能氧化角鲨烯环化酶，产生一种夹竹桃烷型三萜，推定为germanicol，以及β - amyrin和lupeol，比例为82:14:4。MdOSC5以95:5的比例将2,3 -氧化角鲨烯环化成芦皮醇和β - amyrin。CYP716A175催化α - amyrin、β - amyrin、luppeol和germanicol的C - 28氧化，产生熊果酸、齐墩果酸、白桦酸和推测的morolic酸。MdOSC1基因表达与熊果酸和齐墩果酸浓度相关，而MdOSC5基因表达与白桦酸及其咖啡酸衍生物浓度相关。在家苹果中鉴定出两个新的多功能三萜合成酶和一个多功能三萜C - 28氧化酶。MdOSC1和MdOSC5是苹果果实三萜合成的关键基因。
Apple (Malus × domestica) accumulates bioactive ursane‐, oleanane‐, and lupane‐type triterpenes in its fruit cuticle, but their biosynthetic pathway is still poorly understood. We used a homology‐based approach to identify and functionally characterize two new oxidosqualene cyclases (MdOSC4 and MdOSC5) and one cytochrome P450 (CYP716A175). The gene expression patterns of these enzymes and of previously described oxidosqualene cyclases were further studied in 20 apple cultivars with contrasting triterpene profiles. MdOSC4 encodes a multifunctional oxidosqualene cyclase producing an oleanane‐type triterpene, putatively identified as germanicol, as well as β‐amyrin and lupeol, in the proportion 82 : 14 : 4. MdOSC5 cyclizes 2,3‐oxidosqualene into lupeol and β‐amyrin at a ratio of 95 : 5. CYP716A175 catalyses the C‐28 oxidation of α‐amyrin, β‐amyrin, lupeol and germanicol, producing ursolic acid, oleanolic acid, betulinic acid, and putatively morolic acid. The gene expression of MdOSC1 was linked to the concentrations of ursolic and oleanolic acid, whereas the expression of MdOSC5 was correlated with the concentrations of betulinic acid and its caffeate derivatives. Two new multifuntional triterpene synthases as well as a multifunctional triterpene C‐28 oxidase were identified in Malus × domestica. This study also suggests that MdOSC1 and MdOSC5 are key genes in apple fruit triterpene biosynthesis.
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影响因子：
5.1
作者：
Gowri, P. Mangala;Radhakrishnan, S. V. S.;Rao, J. Madhusudana
通讯作者：
Rao, J. Madhusudana
影响因子：
6.1
作者：
Andre, Christelle M.;Greenwood, Jeffrey M.;Laing, William A.
通讯作者：
Laing, William A.
影响因子：
5.1
作者：
Legay, Sylvain;Guerriero, Gea;Hausman, Jean-Francois
通讯作者：
Hausman, Jean-Francois
影响因子：
15
作者：
Kushiro, T;Shibuya, M;Ebizuka, Y
通讯作者：
Ebizuka, Y
影响因子：
3.7
作者：
Gas-Pascual E;Berna A;Bach TJ;Schaller H
通讯作者：
Schaller H