25-Hydroxycholesterol Effect on Membrane Structure and Mechanical Properties.
25-Hydroxycholesterol Effect on Membrane Structure and Mechanical Properties.
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DOI：
10.3390/ijms22052574
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发表时间：
2021-03-04
影响因子：
5.6
通讯作者：
Santos NC
中科院分区：
文献类型：
--
作者：
Domingues MM;Gomes B;Hollmann A;Santos NC
胆固醇负责质膜的可塑性，并参与生理和病理生理反应。胆固醇稳态由氧化甾醇（例如 25-羟基胆固醇）调节。膜水平上 25-羟基胆固醇的存在已被证明会干扰多种病毒进入其靶细胞。我们使用原子力显微镜来评估 25-羟基胆固醇对具有受控脂质成分的支撑脂质双层的不同性质的影响。特别是，我们发现 25-羟基胆固醇抑制胆固醇的脂质凝结作用，使双层的刚性降低。这项研究表明，质膜中包含 25-羟基胆固醇或将其部分胆固醇含量转化为 25-羟基胆固醇会导致富含鞘磷脂 (SM) 的结构域发生形态改变，并促进脂质堆积的不均匀性。这些变化最终导致膜刚度变化。
Cholesterol is responsible for the plasticity of plasma membranes and is involved in physiological and pathophysiological responses. Cholesterol homeostasis is regulated by oxysterols, such as 25-hydroxycholesterol. The presence of 25-hydroxycholesterol at the membrane level has been shown to interfere with several viruses’ entry into their target cells. We used atomic force microscopy to assess the effect of 25-hydroxycholesterol on different properties of supported lipid bilayers with controlled lipid compositions. In particular, we showed that 25-hydroxycholesterol inhibits the lipid-condensing effects of cholesterol, rendering the bilayers less rigid. This study indicates that the inclusion of 25-hydroxycholesterol in plasma membranes or the conversion of part of their cholesterol content into 25-hydroxycholesterol leads to morphological alterations of the sphingomyelin (SM)-enriched domains and promotes lipid packing inhomogeneities. These changes culminate in membrane stiffness variations.
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影响因子：
3.4
作者：
Gomes, Barbara;Goncalves, Sonia;Santos, Nuno C.
通讯作者：
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影响因子：
3.3
作者：
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影响因子：
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BECHHOEFER, J
影响因子：
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影响因子：
3.4
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