Tracking the Initial Diffusion of SARS-CoV-2 Omicron Variant in Italy by RT-PCR and Comparison with Alpha and Delta Variants Spreading.
Tracking the Initial Diffusion of SARS-CoV-2 Omicron Variant in Italy by RT-PCR and Comparison with Alpha and Delta Variants Spreading.
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DOI：
10.3390/diagnostics12020467
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发表时间：
2022-02-11
期刊：
影响因子：
--
通讯作者：
Giardina E
中科院分区：
文献类型：
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作者：
Caputo V;Calvino G;Strafella C;Termine A;Fabrizio C;Trastulli G;Ingrascì A;Peconi C;Bardini S;Rossini A;Salvia A;Borsellino G;Battistini L;Caltagirone C;Cascella R;Giardina E
欧米克隆SARS-CoV-2变种的出现引起了全球公共卫生关注，提高了改进快速监测战略的必要性。本手稿旨在提供证据，证明在意大利，用于常规检测SARS-CoV-2感染的诊断试剂盒是一种具有成本效益的追踪欧米克隆变异的方法。该研究是在患者鼻咽拭子提取的RNA样本上进行的。这些样本使用TaqPath COVID-19 RT PCR CE IVD试剂盒进行RT-PCR检测。使用非参数检验和多项式模型拟合来比较Alpha， Delta和Omicron变体的传播。对感兴趣的样本进行了正确的扩增，并显示存在S基因靶失败，这表明这些患者携带了Omicron变体。与我们的队列中的Alpha和Delta变体相比，发现扩散趋势明显不同且更快。总的来说，这些结果强调了S基因靶失败是一个非常有用的工具，可以在第一次检测后的三周内立即和廉价地跟踪Omicron变异。因此，我们的方法可以作为一线筛查，减少监测策略的时间和成本，促进对COVID-19的预防和应对措施的管理。
The emergence of the Omicron SARS-CoV-2 variant caused public health concerns worldwide, raising the need for the improvement of rapid monitoring strategies. The present manuscript aimed at providing evidence of the utility of a diagnostic kit for the routine testing of SARS-CoV-2 infection as a cost-effective method for tracking the Omicron variant in Italy. The study was conducted on patients’ naso-oropharyngeal-swab-derived RNA samples. These samples were subjected to RT-PCR using the TaqPath COVID-19 RT PCR CE IVD kit. Nonparametric testing and polynomial models fitting were used to compare the spreading of Alpha, Delta and Omicron variants. The samples of interest were correctly amplified and displayed the presence of S gene-target failure, suggesting that these patients carry the Omicron variant. The trend of diffusion was found to be significantly different and more rapid compared with that of the Alpha and Delta variants in our cohorts. Overall, these results highlight that the S gene target failure was a very useful tool for the immediate and inexpensive tracking of Omicron variant in the three weeks from the first detection. Thus, our approach could be used as a first-line screening to reduce the time and costs of monitoring strategies, facilitating the management of preventive and counteracting measures against COVID-19.
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DOI：
10.1016/j.ijid.2021.04.048
发表时间：
2021-07
期刊：
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
影响因子：
--
作者：
Caputo V;Bax C;Colantoni L;Peconi C;Termine A;Fabrizio C;Calvino G;Luzzi L;Panunzi GG;Fusco C;Strafella C;Cascella R;Battistini L;Caltagirone C;Salvia A;Sancesario G;Giardina E
通讯作者：
Giardina E
影响因子：
--
作者：
Caputo V;Termine A;Fabrizio C;Calvino G;Luzzi L;Fusco C;Ingrascì A;Peconi C;D'Alessio R;Mihali S;Trastulli G;Megalizzi D;Cascella R;Rossini A;Salvia A;Strafella C;Giardina E
通讯作者：
Giardina E
SARS-CoV-2 Omicron 变种：特征和预防
影响因子：
9.9
作者：
He X;Hong W;Pan X;Lu G;Wei X
通讯作者：
Wei X
影响因子：
7.3
作者：
Strafella C;Caputo V;Guerrera G;Termine A;Fabrizio C;Cascella R;Picozza M;Caltagirone C;Rossini A;Balice MP;Salvia A;Battistini L;Borsellino G;Giardina E
通讯作者：
Giardina E
影响因子：
5.4
作者：
Mair, Patrick;Wilcox, Rand
通讯作者：
Wilcox, Rand