Intracellular pH regulation: characterization and functional investigation of H(+) transporters in Stylophora pistillata.
Intracellular pH regulation: characterization and functional investigation of H(+) transporters in Stylophora pistillata.
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DOI：
10.1186/s12860-021-00353-x
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发表时间：
2021-03-08
影响因子：
2.8
通讯作者：
Zoccola D
中科院分区：
文献类型：
--
作者：
Capasso L;Ganot P;Planas-Bielsa V;Tambutté S;Zoccola D
由于生理和环境过程，造礁珊瑚经常经历细胞内和细胞外H+浓度（[H+]）的变化。[H+]的严格控制是维持珊瑚细胞内稳态酸碱平衡所必需的，并通过调节细胞内pH值（pHi）来实现。这项任务是特别具有挑战性的造礁珊瑚共享的内共生关系与光合甲藻（共生藻科），这显着影响珊瑚细胞的pH值。尽管它们的重要性，pH值调节蛋白参与的稳态酸碱平衡几乎没有研究珊瑚。在这里，我们报告在珊瑚Stylophora pistillata的基因组结构，结构域拓扑结构和三个主要的H+转运蛋白家族，已知发挥作用的动物细胞的细胞内pH值调节的一个完整的表征;我们研究了他们的组织特异性表达模式，并评估了海水酸化对他们的表达水平的影响。我们鉴定了钠/氢交换器（SLC 9）、液泡型产电H+-ATP水解酶（V-ATP酶）和电压门控质子通道（HvCN）家族成员。雌蕊此外，我们确定了一个新的HvCN基因家族的刺胞动物亚类Hexacorallia，以前没有在任何物种中描述。我们还确定了有助于H+转运蛋白底物特异性，蛋白质功能和调节的关键残基。最后，我们证明了这些蛋白质中的一些具有不同的组织表达模式，并且大多数不受海水酸化的影响。在这项研究中，我们提供了第一个表征的H+转运蛋白，可能有助于在珊瑚细胞的稳态酸碱平衡。这项工作将丰富珊瑚生物学的基本方面的知识，并对我们了解珊瑚如何调节其细胞内环境具有重要意义。在线版本包含补充材料，可通过10.1186/s12860-021-00353-x获得。
Reef-building corals regularly experience changes in intra- and extracellular H+ concentrations ([H+]) due to physiological and environmental processes. Stringent control of [H+] is required to maintain the homeostatic acid-base balance in coral cells and is achieved through the regulation of intracellular pH (pHi). This task is especially challenging for reef-building corals that share an endosymbiotic relationship with photosynthetic dinoflagellates (family Symbiodinaceae), which significantly affect the pHi of coral cells. Despite their importance, the pH regulatory proteins involved in the homeostatic acid-base balance have been scarcely investigated in corals. Here, we report in the coral Stylophora pistillata a full characterization of the genomic structure, domain topology and phylogeny of three major H+ transporter families that are known to play a role in the intracellular pH regulation of animal cells; we investigated their tissue-specific expression patterns and assessed the effect of seawater acidification on their expression levels. We identified members of the Na+/H+ exchanger (SLC9), vacuolar-type electrogenic H+-ATP hydrolase (V-ATPase) and voltage-gated proton channel (HvCN) families in the genome and transcriptome of S. pistillata. In addition, we identified a novel member of the HvCN gene family in the cnidarian subclass Hexacorallia that has not been previously described in any species. We also identified key residues that contribute to H+ transporter substrate specificity, protein function and regulation. Last, we demonstrated that some of these proteins have different tissue expression patterns, and most are unaffected by exposure to seawater acidification. In this study, we provide the first characterization of H+ transporters that might contribute to the homeostatic acid-base balance in coral cells. This work will enrich the knowledge of the basic aspects of coral biology and has important implications for our understanding of how corals regulate their intracellular environment. The online version contains supplementary material available at 10.1186/s12860-021-00353-x.
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