The isolation of novel mesenchymal stromal cell chemotactic factors from the conditioned medium of tumor cells.
The isolation of novel mesenchymal stromal cell chemotactic factors from the conditioned medium of tumor cells.
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DOI：
10.1016/j.yexcr.2008.07.028
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发表时间：
2008-10-15
影响因子：
3.7
通讯作者：
Glod J
中科院分区：
文献类型：
--
作者：
Lin SY;Yang J;Everett AD;Clevenger CV;Koneru M;Mishra PJ;Kamen B;Banerjee D;Glod J
骨髓间充质干细胞（MSCs）定位于实体瘤。确定调节这一过程的信号传导机制对于理解MSC在肿瘤生长中的作用非常重要。使用色谱和电喷雾串联质谱的组合，我们已经确定了新的可溶性信号分子，诱导MSC趋化性存在于乳腺癌细胞系MDA-MB 231的条件培养基。以前的工作已经采用调查策略，使用ELISA测定，以确定已知的趋化因子，促进MSC趋化性。虽然这些研究为影响MSC行为的细胞间信号提供了有价值的见解，但使用这些方法可能会忽略许多描述不太清楚但潜在重要的可溶性信号分子。通过柱层析的不太直接的方法，我们已经确定了新的候选MSC趋化肽。然后进一步表征了两种蛋白质，亲环素B和肝癌衍生生长因子，并显示其促进MSC趋化性。
Bone marrow-derived mesenchymal stromal cells (MSCs) localize to solid tumors. Defining the signaling mechanisms that regulate this process is important to understanding the role of MSCs in tumor growth. Using a combination of chromatography and electrospray tandem mass spectrometry we have identified novel soluble signaling molecules that induce MSC chemotaxis present in conditioned medium of the breast carcinoma cell line MDA-MB231. Previous work has employed survey strategies using ELISA assay to identify known chemokines that promote MSC chemotaxis. While these studies provide valuable insights into the intercellular signals that impact MSC behavior, many less well-described, but potentially important soluble signaling molecules could be overlooked using these methods. Through the less directed method of column chromatography we have identified novel candidate MSC chemotactic peptides. Two proteins, cyclophilin B and hepatoma-derived growth factor were then further characterized and shown to promote MSC chemotaxis.
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影响因子：
11.2
作者：
Nakamizo, A;Marini, F;Lang, FF
通讯作者：
Lang, FF
影响因子：
2.9
作者：
Kuschert, GSV;Coulin, F;Wells, TNC
通讯作者：
Wells, TNC
影响因子：
2.9
作者：
Hoogewerf, AJ;Kuschert, GSV;Wells, TNC
通讯作者：
Wells, TNC
影响因子：
4.8
作者：
Fernandez, M;Simon, V;Minguell, JJ
通讯作者：
Minguell, JJ
DOI：
10.1016/j.bbrc.2005.01.092
发表时间：
2005-04-01
影响因子：
3.1
作者：
Kim, SJ;Cho, HH;Jung, JS
通讯作者：
Jung, JS