Characterization of co-metabolic biodegradation of methyl tert-butyl ether by a Acinetobacter sp. strain.
Characterization of co-metabolic biodegradation of methyl tert-butyl ether by a Acinetobacter sp. strain.
复制标题
不动杆菌对甲基叔丁基醚的共代谢生物降解的表征。
作者：
甲基叔丁基醚（MTBE）是一种常见的地下水污染物，共代谢生物修复是一种很有前途的去除方法。本论文分离到一株能共代谢降解甲基叔丁基醚的细菌，经16 S rRNA基因测序分析，将其命名为不动杆菌SL 3。菌株SL 3能在正构烷烃（C5-C8）上生长，并伴随着MTBE的共代谢。正烷烃底物中存在的碳数显著影响MTBE的降解速率和叔丁醇（TBA）的积累，正辛烷导致较高的MTBE降解速率（Vmax = 36.7 nmol min−1 mg protein −1，Ks = 6.4 mmol L−1）和较低的TBA积累速率。在补料分批反应器中的降解实验表明，在0.1-0.5 mmol L-1范围内的九轮MTBE补充后，不动杆菌属菌株SL 3对MTBE的降解效率没有显示出明显的下降。本文的研究结果揭示了不动杆菌SL 3通过共代谢对MTBE进行生物修复的良好特性，并为开发新的MTBE去除技术奠定了基础。
Co-metabolic bioremediation is a promising approach for the elimination of methyl tert-butyl ether (MTBE), which is a common pollutant found worldwide in ground water. In this paper, a bacterial strain able to co-metabolically degrade MTBE was isolated and named as Acinetobacter sp. SL3 based on 16S rRNA gene sequencing analysis. Strain SL3 could grow on n-alkanes (C5–C8) accompanied with the co-metabolic degradation of MTBE. The number of carbons present in the n-alkane substrate significantly influenced the degradation rate of MTBE and accumulation of tert-butyl alcohol (TBA), with n-octane resulting in a higher MTBE degradation rate (Vmax = 36.7 nmol min−1 mgprotein−1, Ks = 6.4 mmol L−1) and lower TBA accumulation rate. A degradation experiment in a fed-batch reactor revealed that the efficiency of MTBE degradation by Acinetobacter sp. strain SL3 did not show an obvious decrease after nine rounds of MTBE replenishment ranging from 0.1–0.5 mmol L−1. The results of this paper reveal the preferable properties of Acinetobacter sp. SL3 for the bioremediation of MTBE via co-metabolism and leads towards the development of new MTBE elimination technologies.
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DOI：
10.1128/genomea.01028-16
发表时间：
2016-09-01
期刊：
GENOME ANNOUNCEMENTS
影响因子：
--
作者：
Lee, Yunmi;Jang, Soojin
通讯作者：
Jang, Soojin
影响因子：
4.2
作者：
Malandain, Cedric;Fayolle-Guichard, Francoise;Vogel, Timothy M.
通讯作者：
Vogel, Timothy M.
DOI：
10.1073/pnas.042263399
发表时间：
2002-02-19
影响因子：
11.1
作者：
de Vries, J;Wackernagel, W
通讯作者：
Wackernagel, W
影响因子：
4.4
作者：
Kostka, Joel E.;Prakash, Om;Huettel, Markus
通讯作者：
Huettel, Markus
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.