Treadmill training stimulates brain-derived neurotrophic factor mRNA expression in motor neurons of the lumbar spinal cord in spinally transected rats.
Treadmill training stimulates brain-derived neurotrophic factor mRNA expression in motor neurons of the lumbar spinal cord in spinally transected rats.
复制标题
DOI：
10.1016/j.neuroscience.2012.08.024
复制
发表时间：
2012-11-08
期刊：
影响因子：
3.3
通讯作者：
de Leon, R. D.
中科院分区：
文献类型：
--
作者：
Joseph, M. S.;Tillakaratne, N. J. K.;de Leon, R. D.
脑源性神经营养因子（BDNF）诱导腰椎回路的可塑性，从而改善脊髓损伤动物的运动恢复。我们研究了是否腰脊髓运动神经元和其他腹角细胞脊髓横断（ST）大鼠刺激产生BDNF mRNA在跑步机训练的反应。大鼠在新生时接受完全脊髓横断（n=20），一个月后，接受4周的低（100步/训练期; n=10）或高（1000步/训练期; n=10）量的机器人辅助跑步机训练。采用非放射性原位杂交和免疫组织化学技术相结合，我们发现BDNF mRNA的表达在HSP 27标记的运动神经元和非运动神经元细胞是更大的1000步/训练后的100步/训练期相比，是类似的BDNF mRNA标记在未训练的完整大鼠。此外，有更多的运动神经元，含有BDNF mRNA标记的进程中的ST大鼠，接受了较高的量的跑台训练。这些结果表明，运动神经元和其他腹角细胞在脊髓横断大鼠合成BDNF的反应跑台训练。这一发现支持了运动神经元突触后释放BDNF促进突触可塑性的机制。
Brain derived neurotrophic factor (BDNF) induces plasticity within the lumbar spinal circuits thereby improving locomotor recovery in spinal cord injured animals. We examined whether lumbar spinal cord motor neurons and other ventral horn cells of spinally transected (ST) rats were stimulated to produce BDNF mRNA in response to treadmill training. Rats received complete spinal cord transections as neonates (n=20) and one month later, received 4 weeks of either a low (100 steps/training session; n=10) or high (1000 steps/training session; n=10) amount of robotic-assisted treadmill training. Using combined non-radioactive in situ hybridization and immunohistochemical techniques, we found BDNF mRNA expression in HSP27-labeled motor neurons and in non-motor neuron cells was greater after 1000 steps/training session compared to the 100 steps/training session and was similar to BDNF mRNA labeling in untrained intact rats. In addition, there were significantly more motor neurons that contained BDNF mRNA labeling within processes in the ST rats that received the higher amount of treadmill training. These findings suggested that motor neurons and other ventral horn cells in spinally transected rats synthesized BDNF in response to treadmill training. The findings support a mechanism by which postsynaptic release of BDNF from motor neurons contributed to synaptic plasticity.
登录
查看更多内容
影响因子：
2.4
作者：
Macias M;Nowicka D;Czupryn A;Sulejczak D;Skup M;Skangiel-Kramska J;Czarkowska-Bauch J
通讯作者：
Czarkowska-Bauch J
影响因子：
6.1
作者：
Dougherty, KD;Dreyfus, CF;Black, IB
通讯作者：
Black, IB
影响因子：
3.2
作者：
Behrman, AL;Lawless-Dixon, AR;Harkema, SJ
通讯作者：
Harkema, SJ
影响因子：
5.3
作者：
SMITH, MA;MAKINO, S;POST, RM
通讯作者：
POST, RM
影响因子：
14.5
作者：
Hutchinson, KJ;Gómez-Pinilla, F;Basso, DM
通讯作者：
Basso, DM