Microbial Upcycling of Waste PET to Adipic Acid.
Microbial Upcycling of Waste PET to Adipic Acid.
复制标题
废弃PET微生物升级回收己二酸。
DOI：
10.1021/acscentsci.3c00414
复制
发表时间：
2023-11-22
影响因子：
18.2
通讯作者：
Wallace, Stephen
中科院分区：
文献类型：
--
作者：
Valenzuela-Ortega, Marcos;Suitor, Jack T.;White, Mirren F. M.;Hinchcliffe, Trevor;Wallace, Stephen
微生物可以通过基因工程改造，将丰富的废物原料转化为增值的小分子，否则将从日益减少的化石资源中制造出来。在此，我们报道了PET塑料废物在大肠杆菌中首次一锅生物升级回收为高产平台石化和尼龙前体己二酸。优化外源基因表达和酶活性可以增加从头途径的通量，并且将整个细胞固定在海藻酸盐水凝胶中可以提高限速的enoate还原酶BcER的稳定性。该途径酶还与工程大肠杆菌DD-2产生的氢气结合，并与生物相容性Pd催化剂结合，使代谢顺式、顺式黏液酸合成己二酸。总之，这些优化导致了从对苯二甲酸到己二酸的一锅转化，包括从工业PET废物和消费后塑料瓶中分离的对苯二甲酸样品。据报道，大肠杆菌将消费后和工业PET样品中的对苯二甲酸盐废物转化为工业化学品和尼龙前体己二酸。
Microorganisms can be genetically engineered to transform abundant waste feedstocks into value-added small molecules that would otherwise be manufactured from diminishing fossil resources. Herein, we report the first one-pot bio-upcycling of PET plastic waste into the prolific platform petrochemical and nylon precursor adipic acid in the bacterium Escherichia coli. Optimizing heterologous gene expression and enzyme activity enabled increased flux through the de novo pathway, and immobilization of whole cells in alginate hydrogels increased the stability of the rate-limiting enoate reductase BcER. The pathway enzymes were also interfaced with hydrogen gas generated by engineered E. coli DD-2 in combination with a biocompatible Pd catalyst to enable adipic acid synthesis from metabolic cis,cis-muconic acid. Together, these optimizations resulted in a one-pot conversion to adipic acid from terephthalic acid, including terephthalate samples isolated from industrial PET waste and a post-consumer plastic bottle. A de novo biosynthesis pathway is reported in the bacterium Escherichia coli to convert terephthalate waste from post-consumer and industrial PET samples into the industrial chemical and nylon precursor adipic acid.
登录
查看更多内容
影响因子：
13.6
作者：
Geyer R;Jambeck JR;Law KL
通讯作者：
Law KL
影响因子：
5.7
作者：
Wei R;Zimmermann W
通讯作者：
Zimmermann W
影响因子：
8.8
作者：
Diao, Jinjin;Hu, Yifeng;Moon, Tae Seok
通讯作者：
Moon, Tae Seok
影响因子：
56.9
作者：
Ravishankara, A. R.;Daniel, John S.;Portmann, Robert W.
通讯作者：
Portmann, Robert W.
影响因子：
5.7
作者：
Dissanayake L;Jayakody LN
通讯作者：
Jayakody LN