Protection of MES23.5 dopaminergic cells by obestatin is mediated by proliferative rather than anti-apoptotic action
Protection of MES23.5 dopaminergic cells by obestatin is mediated by proliferative rather than anti-apoptotic action
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肥胖抑制素对 MES23.5 多巴胺能细胞的保护是通过增殖作用而非抗凋亡作用介导的
DOI：
10.1007/s12264-013-1405-0
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发表时间：
2014-01
影响因子：
5.6
通讯作者：
Jiang Hong
中科院分区：
文献类型：
--
作者：
Shen Xiao-Li;Jia Feng-Ju;Song Ning;Xie Jun-Xia;Jiang Hong
奥斯汀是一种内源性多肽，与Ghrelin具有共同的前体。本研究旨在探讨1-甲基-4-苯基吡啶(MPP+)对MES23.5多巴胺能细胞是否具有保护作用。采用四甲基偶氮唑蓝比色法检测细胞存活率，再用2 0 0−13~10−6μ/L(10−13~10−6 m ol/L)处理0、6、12、2 4h，再与2 0 0 m ol/L MPP+孵育12或2 4h。流式细胞仪检测caspase-3活性和线粒体膜电位。免疫印迹法检测增殖细胞核抗原(PCNA)蛋白水平。奥斯汀(10−13~10−7mol/L)可阻断甚至逆转MPP+诱导的MES23.5细胞存活率下降，但对MPP+诱导的线粒体跨膜电位崩溃和caspase3激活无影响。单独应用时，奥贝他汀可提高存活率。10−7、10−9、10−11和10−13mol/L处理12h后，增殖细胞核抗原表达明显升高。
Obestatin is an endogenous peptide sharing a precursor with ghrelin. This study aims to investigate whether and how obestatin protects MES23.5 dopaminergic cells against 1-methyl-4-phenylpyridinium (MPP+)-induced neurotoxicity. MES23.5 cells were pretreated with obestatin (10−13–10−6 mol/L) for 20 min prior to incubation with 200 μmol/L MPP+ for 12 or 24 h, or treated with obestatin alone (10−13 to 10−6 mol/L) for 0, 6, 12, and 24 h. The methyl thiazolyl tetrazolium (MTT) assay was used to measure cell viability. Flow cytometry was used to measure the caspase-3 activity and the mitochondrial transmembrane potential. Proliferating cell nuclear antigen (PCNA) protein levels were determined by Western blotting. Obestatin (10−13 to 10−7 mol/L) pretreatment blocked or even reversed the MPP+-induced reduction of viability in MES23.5 cells, but had no effect on MPP+-induced mitochondrial transmembrane potential collapse and caspase-3 activation. When applied alone, obestatin increased viability. Elevated PCNA levels occurred with 10−7, 10−9, 10−11 and 10−13 mol/L obestatin treatment for 12 h.
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二价金属转运蛋白 1 的上调参与 1-甲基-4-苯基吡啶 (MPP) 诱导的 MES23.5 细胞凋亡
DOI：
10.1016/j.neurobiolaging.2007.11.025
发表时间：
2009-09
期刊：
Neurobiol Aging
影响因子：
--
作者：
Xie Junxia;Zhang Shuzhen;Song N;Wang Jun;Jiang Hong
通讯作者：
Jiang Hong
影响因子：
7.7
作者：
R. Granata;F. Settanni;David J. Gallo;L. Trovato;L. Biancone;V. Cantaluppi;R. Nano;M. Annunziata-
通讯作者：
R. Granata;F. Settanni;David J. Gallo;L. Trovato;L. Biancone;V. Cantaluppi;R. Nano;M. Annunziata-
影响因子：
3.4
作者：
LEONARDI, E;GIRLANDO, S;BARBARESCHI, M
通讯作者：
BARBARESCHI, M
影响因子：
4.4
作者：
Teresa R. Sanelli;Wei-wen Ge;C. Leystra‐Lantz;M. Strong
通讯作者：
Teresa R. Sanelli;Wei-wen Ge;C. Leystra‐Lantz;M. Strong
DOI：
10.1523/jneurosci.12-09-03392.1992
发表时间：
1992-09
期刊：
--
影响因子：
--
作者：
G. Crawford;WD Le;R.Glenn Smith;WJ Xie;E. Stefani;Sarit Appel
通讯作者：
G. Crawford;WD Le;R.Glenn Smith;WJ Xie;E. Stefani;Sarit Appel