Rho GTPase ROP1 Interactome Analysis Reveals Novel ROP1-Associated Pathways for Pollen Tube Polar Growth in Arabidopsis.
Rho GTPase ROP1 Interactome Analysis Reveals Novel ROP1-Associated Pathways for Pollen Tube Polar Growth in Arabidopsis.
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Rho GTPase ROP1 相互作用组分析揭示了拟南芥花粉管极性生长的 ROP1 相关新途径
DOI：
10.3390/ijms21197033
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发表时间：
2020-09-24
影响因子：
5.6
通讯作者：
Yang Z
中科院分区：
文献类型：
--
作者：
Li H;Hu J;Pang J;Zhao L;Yang B;Kang X;Wang A;Xu T;Yang Z
ROP（Rho-like GTPases from plants）GTPases是植物花粉管和其他细胞极性生长的关键调控因子。然而，ROP GTPases如何调节以及它们如何控制极性生长仍有待充分了解。为了获得新的见解ROP依赖的机制，极性细胞生长的基础上，我们的特点是ROP 1 GTbinds的相互作用，控制拟南芥花粉管（PT）顶端生长，极性细胞生长的极端形式。我们建立了一种高效的拟南芥花粉管液体培养方法，用于ROP 1相关蛋白的免疫沉淀/质谱鉴定。从拟南芥花粉管中的ROP 1相互作用组中共分离出654个候选物，GO（Gene Ontology）分类和途径分析揭示了除了已知的ROP 1依赖性途径之外，还存在多个未表征的ROP 1依赖性过程，包括翻译、细胞壁修饰、转录后修饰和离子稳态。ROP 1相互作用基因组数据进一步得到了候选相互作用基因在高度成熟花粉中的共表达的支持，其中在25%（8/32）的候选突变基因中发现了PT萌发和生长缺陷。综上所述，我们的工作揭示了有价值的信息，识别和功能阐明ROP相关蛋白的极性生长的调节，并提供了一个可靠的参考，以确定在未来的极性细胞生长的关键调节剂。
ROP (Rho-like GTPases from plants) GTPases are polarly localized key regulators of polar growth in pollen tubes and other cells in plants. However, how ROP GTPases are regulated and how they control polar growth remains to be fully understood. To gain new insights into ROP-dependent mechanisms underlying polar cell growth, we characterized the interactome of ROP1 GTPase that controls Arabidopsis pollen tube (PT) tip growth, an extreme form of polar cell growth. We established an efficient method for culturing Arabidopsis pollen tubes in liquid medium, which was used for immunoprecipitation/mass spectrometry-based identification of ROP1-associated proteins. A total of 654 candidates were isolated from the ROP1 interactome in Arabidopsis pollen tubes, and GO (Gene Ontology) classification and pathway analysis revealed multiple uncharacterized ROP1-dependent processes including translation, cell wall modification, post transcriptional modification, and ion homeostasis, in addition to known ROP1-dependent pathways. The ROP1-interactome data was further supported by the co-expression of the candidate interactors in highly mature pollen with PT germination and growth defects being discovered in 25% (8/32) of the candidate mutant genes. Taken together, our work uncovers valuable information for the identification and functional elucidation of ROP-associated proteins in the regulation of polar growth, and provides a reliable reference to identify critical regulators of polar cell growth in the future.
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影响因子：
7
作者：
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通讯作者：
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影响因子：
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通讯作者：
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