Proliferative activity in coronary atherectomy tissue. Clinical, histopathologic, and immunohistochemical correlates.
Proliferative activity in coronary atherectomy tissue. Clinical, histopathologic, and immunohistochemical correlates.
复制标题
冠状动脉粥样斑块切除组织中的增殖活性。
作者：
Allen J. Taylor;A. Farb;D. Angello;L. R. Burwell;L. R. Burwell;R. Virmani;R. Virmani
研究目标
尽管细胞增殖被认为是导致冠状动脉介入治疗后再狭窄的主要过程之一，但对于动脉粥样硬化组织中细胞复制的程度仍存在争议。因此，我们试图调查通过定向冠状动脉动脉粥样硬化术(DCA)获得的动脉粥样硬化组织中增殖活性的水平和临床病理相关性。
设计
前瞻性观察性研究。
设置
三级护理转诊医院。
病人
对37个病变(原发灶26个，再狭窄灶11个)的DCA标本进行增殖细胞核抗原和碱性成纤维细胞生长因子的单标记免疫组织化学染色。
结果
再狭窄组织比原发组织更有可能含有内膜增生区(vs23%；p<；0.03)。而增殖细胞核抗原在原发灶和再狭窄灶中的阳性染色频率相似(25vs30%；p=NS)，每片阳性细胞的平均百分比在两组中相似。BFGF阳性表达20例(61%)，且多见于再狭窄病变(80例vs52%；p=0.25)。但bFGF与增殖细胞核抗原的表达无相关性或共存。我们没有发现临床病理与临床结果的相关性。
结论
从接受冠状动脉介入治疗的患者获得的原发和再狭窄动脉粥样硬化组织中，通过增殖细胞核抗原的表达来衡量细胞复制是以不同的模式发生的。
STUDY OBJECTIVE
Although cellular proliferation is considered one of the dominant processes leading to restenosis following coronary intervention, controversy exists over the extent of cellular replication in atherosclerotic tissue. Accordingly, we sought to investigate the level and clinicopathologic correlates of proliferative activity in atherosclerotic tissue obtained via directional coronary atherectomy (DCA).
DESIGN
Prospective observational study.
SETTING
Tertiary care referral hospital.
PATIENTS
Specimens retrieved via DCA from 37 lesions (primary, 26; restenosis, 11) were studied using single-label immunohistochemical staining for the proliferating cell nuclear antigen and basic fibroblast growth factor (bFGF).
RESULTS
Restenosis tissue was significantly more likely than primary tissue to contain areas of intimal hyperplasia (64 vs 23%; p < 0.03). However, the frequency of positive staining for proliferating cell nuclear antigen (PCNA) was similar in primary and restenosis lesions (25 vs 30%; p = NS), and the mean percentage of positive cells per slide was similar in the two groups. Positive immunostaining for bFGF was present in 20 lesions (61%), and tended to be more frequently seen in restenotic lesions (80 vs 52%; p = 0.25). However, there was no correlation or colocalization between immunostaining for bFGF and proliferating cell nuclear antigen. We found no clinicopathologic correlations with respect to clinical outcome.
CONCLUSIONS
Cellular replication, as measured by expression of the PCNA, occurs in a heterogeneous pattern in both primary and restenotic atherosclerotic tissue obtained from patients undergoing coronary intervention.
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DOI：
10.1073/pnas.87.12.4600
发表时间：
1990-06-01
影响因子：
11.1
作者：
GORDON, D;REIDY, MA;SCHWARTZ, SM
通讯作者：
SCHWARTZ, SM
影响因子：
19.9
作者：
Tong F
通讯作者：
Tong F
影响因子：
20.1
作者：
LINDNER, V;LAPPI, DA;REIDY, MA
通讯作者：
REIDY, MA
影响因子：
20.1
作者：
OBRIEN, ER;ALPERS, CE;SCHWARTZ, SM
通讯作者：
SCHWARTZ, SM
DOI：
10.1161/01.atv.10.6.1082
发表时间：
1990-11-01
期刊：
ARTERIOSCLEROSIS
影响因子：
--
作者：
FINGERLE, J;AU, YPT;REIDY, MA
通讯作者：
REIDY, MA