Fusarium pathogenesis investigated using Galleria mellonella as a heterologous host.
Fusarium pathogenesis investigated using Galleria mellonella as a heterologous host.
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使用梅洛尼氏菌作为异源宿主进行了研究。
DOI：
10.1016/j.funbio.2011.09.005
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发表时间：
2011-12
期刊：
影响因子：
2.5
通讯作者：
Mylonakis, Eleftherios
中科院分区：
文献类型：
--
作者：
Coleman, Jeffrey J.;Muhammed, Maged;Kasperkovitz, Pia V.;Vyas, Jatin M.;Mylonakis, Eleftherios
镰刀菌属真菌的成员能够表现为多种临床感染，最常见于免疫功能低下的患者。为了更好地了解真菌和宿主之间的相互作用，我们已经开发了大蜡螟的幼虫，大蜡螟，作为镰刀菌的异源宿主。当分生孢子被注入到这个鳞翅目系统的血腔中时，真菌的临床和环境分离物都能够在37°C下杀死幼虫，尽管在30°C下孵育时杀死发生得更快。这种杀伤取决于除温度之外的几个其他因素，包括镰刀菌属菌株、注射的分生孢子数量和分生孢子形态，其中大分生孢子比它们的小分生孢子对应物更具毒性。对镰刀菌的杀灭率存在一定的相关性。在G.梅隆菌和鼠模型。体内研究表明G.大蜡螟血细胞最初能够吞噬两种分生孢子形态。共和党大蜡螟系统也被用来评估抗真菌剂，并且黄曲霉素B能够赋予镰刀菌感染的幼虫存活率的显著增加。共和党大蜡螟-镰刀菌致病力系统研究表明，是相似的，无论分离物的来源，哺乳动物的吸热是镰刀菌感染的主要威慑，因此提供了一个合适的替代哺乳动物模型来研究宿主和这种日益重要的真菌病原体之间的相互作用。
Members of the fungal genus Fusarium are capable of manifesting in a multitude of clinical infections, most commonly in immunocompromised patients. In order to better understand the interaction between the fungus and host, we have developed the larvae of the greater wax moth, Galleria mellonella, as a heterologous host for fusaria. When conidia are injected into the hemocoel of this Lepidopteran system, both clinical and environmental isolates of the fungus are able to kill the larvae at 37°C, although killing occurs more rapidly when incubated at 30°C. This killing was dependent on several other factors besides temperature, including the Fusarium strain, the number of conidia injected, and the conidia morphology, where macroconidia are more virulent than their microconidia counterpart. There was a correlation in the killing rate of Fusarium spp. when evaluated in G. mellonella and a murine model. In vivo studies indicated G. mellonella hemocytes were capable of initially phagocytosing both conidial morphologies. The G. mellonella system was also used to evaluate antifungal agents, and amphotericin B was able to confer a significant increase in survival to Fusarium infected-larvae. The G. mellonella-Fusarium pathogenicity system revealed that virulence of Fusarium spp. is similar, regardless of the origin of the isolate, and that mammalian endothermy is a major deterrent for Fusarium infection and therefore provides a suitable alternative to mammalian models to investigate the interaction between the host and this increasingly important fungal pathogen.
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影响因子：
3
作者：
Mayayo, E;Pujol, I;Guarro, J
通讯作者：
Guarro, J
DOI：
10.1016/j.ijfoodmicro.2007.07.024
发表时间：
2007-10-20
影响因子：
5.4
作者：
Desjardins, A. E.;Proctor, R. H.
通讯作者：
Proctor, R. H.
影响因子：
2.8
作者：
Allen, TW;Enebak, SA;Carey, WA
通讯作者：
Carey, WA
烟曲霉的分生物色突变体对异源昆虫宿主Galleria mellonella具有高度致病性。
影响因子：
3.7
作者：
Jackson JC;Higgins LA;Lin X
通讯作者：
Lin X
影响因子：
4.5
作者：
Coleman JJ;Rounsley SD;Rodriguez-Carres M;Kuo A;Wasmann CC;Grimwood J;Schmutz J;Taga M;White GJ;Zhou S;Schwartz DC;Freitag M;Ma LJ;Danchin EG;Henrissat B;Coutinho PM;Nelson DR;Straney D;Napoli CA;Barker BM;Gribskov M;Rep M;Kroken S;Molnár I;Rensing C;Kennell JC;Zamora J;Farman ML;Selker EU;Salamov A;Shapiro H;Pangilinan J;Lindquist E;Lamers C;Grigoriev IV;Geiser DM;Covert SF;Temporini E;Vanetten HD
通讯作者：
Vanetten HD