Biointeractions of Herbicide Atrazine with Human Serum Albumin: UV-Vis, Fluorescence and Circular Dichroism Approaches.
Biointeractions of Herbicide Atrazine with Human Serum Albumin: UV-Vis, Fluorescence and Circular Dichroism Approaches.
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除草剂莠去津与人血清白蛋白的生物相互作用：紫外-可见光、荧光和圆二色性方法
DOI：
10.3390/ijerph15010116
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发表时间：
2018-01-11
影响因子：
--
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Zhu M;Wang L;Wang Y;Zhou J;Ding J;Li W;Xin Y;Fan S;Wang Z;Wang Y
除草剂阿特拉津在地球仪上广泛使用，引起了人们的极大关注。为了研究其在人体内的潜在毒性，选择人血清白蛋白（HSA）作为模型蛋白。采用稳态荧光光谱、同步荧光光谱、紫外-可见光谱、三维荧光光谱和圆二色光谱研究了阿特拉津与人血清白蛋白的相互作用。阿特拉津通过静态猝灭机制猝灭人血清白蛋白的内源荧光。在两个激发波长（280和295 nm）下的荧光光谱表明，色氨酸残基对HSA的荧光猝灭有重要贡献。阿特拉津与人血清白蛋白结合，引起人血清白蛋白构象和二级结构的变化，引起能量转移。热力学参数表明，这种结合是自发的。静电相互作用在阿特拉津与HSA的结合中起主要作用。一个阿特拉津分子只能与一个HSA分子结合形成复合物，并且阿特拉津分子结合在HSA的位点II（亚结构域IIIA）。这项研究进一步了解阿特拉津在分子水平上对人类的潜在影响。
The herbicide atrazine is widely used across the globe, which is a great concern. To investigate its potential toxicity in the human body, human serum albumin (HSA) was selected as a model protein. The interaction between atrazine and HSA was investigated using steady-state fluorescence spectroscopy, synchronous fluorescence spectroscopy, UV-Vis spectroscopy, three-dimensional (3D) fluorescence spectroscopy and circular dichroism (CD) spectroscopy. The intrinsic fluorescence of HSA was quenched by the atrazine through a static quenching mechanism. Fluorescence spectra at two excitation wavelengths (280 and 295 nm) showed that the fluorescence quenched in HSA was mainly contributed to by tryptophan residues. In addition, the atrazine bound to HSA, which induced changes in the conformation and secondary structure of HSA and caused an energy transfer. Thermodynamic parameters revealed that this binding is spontaneous. Moreover, electrostatic interactions play a major role in the combination of atrazine and HSA. One atrazine molecule can only bind to one HSA molecule to form a complex, and the atrazine molecule is bound at site II (subdomain IIIA) of HSA. This study furthers the understanding of the potential effects posed by atrazine on humans at the molecular level.
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影响因子：
4.6
作者：
Ding F;Wang M;Zhang S;Ai X
通讯作者：
Ai X
影响因子：
4.8
作者：
Forster, Marie-Therese;Hoecker, Alexander Claudius;Weise, Lutz Martin
通讯作者：
Weise, Lutz Martin
影响因子：
4.3
作者：
Cavas, Tolga
通讯作者：
Cavas, Tolga
DOI：
10.3964/j.issn.1000-0593(2009)11-2998-05
发表时间：
2009-11-01
影响因子：
0.7
作者：
Duan Yun-qing;Lei Huan-gui;Duan Zhi-qing
通讯作者：
Duan Zhi-qing
DOI：
10.3390/ijerph13070640
发表时间：
2016-06-28
影响因子：
--
作者：
Dong J;Dai W;Xu J;Li S
通讯作者：
Li S