Changes in SBPase activity influence photosynthetic capacity, growth, and tolerance to chilling stress in transgenic tomato plants.
Changes in SBPase activity influence photosynthetic capacity, growth, and tolerance to chilling stress in transgenic tomato plants.
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DOI：
10.1038/srep32741
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发表时间：
2016-09-02
影响因子：
4.6
通讯作者：
Ai X
中科院分区：
文献类型：
--
作者：
Ding F;Wang M;Zhang S;Ai X
sedoheptulose - 1,7 -双磷酸酶（SBPase）是卡尔文循环中参与光合固碳的重要酶。本文报道了SBPase活性变化对SBPase反义和转义番茄植株光合作用、生长发育和抗寒性的影响。在SBPase活性增加的转基因植株中，光合速率增加，同时蔗糖和淀粉积累明显增加。与同等野生型番茄植株相比，SBPase阳性植株的总生物量和叶面积增加，反义植株的总生物量和叶面积减少。在低温胁迫下，与SBPase活性降低的植株相比，SBPase活性升高的植株具有更强的耐冷性，表现为电解质泄漏减少，光合能力增加，光系统II RuBP再生速率和量子效率提高。总的来说，我们的数据表明，更高水平的SBPase活性有利于番茄植株的光合作用、生长和耐冷性。这项工作也为卡尔文循环中的单个酶提供了一个案例研究，可以作为基因工程的有用靶标，以提高作物的产量和耐受性。
Sedoheptulose-1, 7-bisphosphatase (SBPase) is an important enzyme involved in photosynthetic carbon fixation in the Calvin cycle. Here, we report the impact of changes in SBPase activity on photosynthesis, growth and development, and chilling tolerance in SBPase antisense and sense transgenic tomato (Solanum lycopersicum) plants. In transgenic plants with increased SBPase activity, photosynthetic rates were increased and in parallel an increase in sucrose and starch accumulation was evident. Total biomass and leaf area were increased in SBPase sense plants, while they were reduced in SBPase antisense plants compared with equivalent wild-type tomato plants. Under chilling stress, when compared with plants with decreased SBPase activity, tomato plants with increased SBPase activity were found to be more chilling tolerant as indicated by reduced electrolyte leakage, increased photosynthetic capacity, and elevated RuBP regeneration rate and quantum efficiency of photosystem II. Collectively, our data suggest that higher level of SBPase activity gives an advantage to photosynthesis, growth and chilling tolerance in tomato plants. This work also provides a case study that an individual enzyme in the Calvin cycle may serve as a useful target for genetic engineering to improve production and stress tolerance in crops.
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