Multidimensional study of the oral microbiome, metabolite, and immunologic environment in peanut allergy.
Multidimensional study of the oral microbiome, metabolite, and immunologic environment in peanut allergy.
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DOI：
10.1016/j.jaci.2021.03.028
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发表时间：
2021-08
期刊：
影响因子：
--
通讯作者：
Bunyavanich S
中科院分区：
文献类型：
--
作者：
Ho HE;Chun Y;Jeong S;Jumreornvong O;Sicherer SH;Bunyavanich S
口腔粘膜是食物抗原、微生物区系和粘膜免疫之间的初始界面，但对食物过敏中口腔宿主环境的动态变化知之甚少。确定与花生过敏相关的口腔微生物、代谢和免疫学特征。我们招募了105名受试者(花生过敏者n=56，健康者n=49)，用16S rRNA测序分析唾液微生物组，用液相色谱/质谱仪测定短链脂肪酸(SCFA)代谢物，用多重分析测定口腔分泌的细胞因子。执行了数据类型内部和跨数据类型的分析。花生过敏个体的口腔微生物群的特征是乳杆菌目、拟杆菌目(普氏杆菌目)和芽孢杆菌属的种类减少，而内氏杆菌属的种类增加。花生过敏受试者独特的口腔微生物群伴随着口腔SCFA水平的显著降低，包括醋酸盐、丁酸盐和丙酸，而IL-4的分泌显著升高。口腔普氏杆菌丰度降低。和Veillonella spp.在花生过敏受试者中，口服SCFA水平降低(FDR<；0.05)和口腔奈瑟菌增多显著相关。与较低的口腔SCFA水平相关(FDR<；0.05)。此外，口服普雷沃特氏菌(Prevotea spp.)Th2刺激因子(IL-33，TSLP)和Th2细胞因子(IL-4，IL-5，IL-13)的局部分泌减少与丰度相关，而口服奈瑟氏菌。丰度与Th2偏斜的口腔免疫环境呈正相关。我们对口腔环境的新的多维分析揭示了与花生过敏的粘膜免疫紊乱相关的不同微生物和代谢特征。我们的发现突出了口腔环境作为一个有兴趣的解剖学位置来研究食物过敏中宿主-微生物组的动态。花生过敏具有独特的口腔微生物、代谢和免疫学特征。口腔普雷沃特氏菌、韦洛氏菌。和奈瑟氏菌(Neisseria spp.)丰度与口服短链脂肪酸水平(醋酸盐、丁酸盐、丙酸)和Th2细胞因子环境的改变有关。
The oral mucosa is the initial interface between food antigens, microbiota and mucosal immunity, yet little is known about oral host-environment dynamics in food allergy. To determine oral microbial, metabolic, and immunologic profiles associated with peanut allergy. We recruited 105 subjects (peanut allergic n=56, healthy subjects n=49) for salivary microbiome profiling using 16S rRNA sequencing, short chain fatty acid (SCFA) metabolite assays using liquid chromatography/mass spectrometry, and measurement of oral secreted cytokines using multiplex assays. Analyses within and across data types were performed. The oral microbiome of peanut-allergic individuals was characterized by reduced species in Lactobacillales, Bacteroidales (Prevotella), and Bacillales, and increased Neisseriales spp. The distinct oral microbiome of peanut-allergic subjects was accompanied by significant reductions of oral SCFA levels, including acetate, butyrate, and propionate, and significant elevation of IL-4 secretion. Decreased abundances of oral Prevotella spp. and Veillonella spp. in peanut-allergic subjects were significantly correlated with reduced oral SCFA levels (FDR<0.05), and increased oral Neisseria spp. was correlated with lower oral SCFA levels (FDR<0.05). Additionally, oral Prevotella spp. abundances were correlated with decreased local secretion of Th2-stimulating epithelial factors (IL-33, TSLP) and Th2 cytokines (IL-4, IL-5, IL-13), while oral Neisseria spp. abundance was positively associated with a Th2-skewed oral immune milieu. Our novel multi-dimensional analysis of the oral environment revealed distinct microbial and metabolic profiles associated with mucosal immune disturbances in peanut allergy. Our findings highlight the oral environment as an anatomical site of interest to examine host-microbiome dynamics in food allergy. Distinct oral microbial, metabolic, and immunologic profiles characterize peanut allergy. Oral Prevotella spp., Veillonella spp. and Neisseria spp. abundances are associated with altered oral short chain fatty acid levels (acetate, butyrate, propionate) and a Th2 cytokine milieu.
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影响因子：
8
作者：
Han H;Roan F;Johnston LK;Smith DE;Bryce PJ;Ziegler SF
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