Metabolomic Characterization Reveals ILF2 and ILF3 Affected Metabolic Adaptions in Esophageal Squamous Cell Carcinoma.
Metabolomic Characterization Reveals ILF2 and ILF3 Affected Metabolic Adaptions in Esophageal Squamous Cell Carcinoma.
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代谢组学表征揭示 ILF2 和 ILF3 影响食管鳞状细胞癌的代谢适应
DOI：
10.3389/fmolb.2021.721990
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发表时间：
2021
影响因子：
5
通讯作者：
Ma Y
中科院分区：
文献类型：
--
作者：
Zang B;Wang W;Wang Y;Li P;Xia T;Liu X;Chen D;Piao HL;Qi H;Ma Y
食管癌（EC）是东方国家常见的恶性肿瘤。然而，对食管鳞状细胞癌（ESCC）中与其他生物因素相关的代谢组学特征的研究有限。据报道，白细胞介素增强子结合因子2（ILF2）和ILF3是双链RNA结合蛋白，与多种恶性肿瘤的发生和发展有关。然而，ILF2和ILF3在ESCC代谢重编程中的潜在功能从未被报道过。本研究旨在促进 ESCC 的代谢特征，并研究 ESCC 组织中与 ILF2 和 ILF3 相关的代谢组学改变。在这里，我们利用 28 名 ESCC 患者的 ESCC 组织和配对癌旁组织，基于液相色谱-质谱法和毛细管电泳-质谱法，鉴定了 112 种差异代谢物，主要富集在磷脂酰胆碱生物合成、脂肪酸代谢和氨基酸代谢途径中。此外，与组织学正常样本相比，EC组织中ILF2和ILF3的表达显着升高，并且与ESCC中的PI3K/AKT和MAPK信号通路密切相关。此外，在ILF2高表达的ESCC组织中，与BCAA代谢途径相关的几种短链酰基肉碱（C3:0、C4:0和C5:0）和参与脂肪酸氧化的长链酰基肉碱（C14:0、C16:0、C16:0-OH和C18:0）明显上调。此外，与相应的癌旁组织相比，ILF3高表达的ESCC组织中涉及糖酵解途径的一系列中间代谢物，包括G6P/F6P、F1,6BP、DHAP、G3P和2,3BPG均显着下调。总的来说，这些发现可能为ILF2和ILF3在ESCC代谢改变过程中的作用提供证据，并为ESCC早期诊断和治疗的发展提供新的见解。需要进一步研究以阐明 ILF2 和 ILF3 分别对酰基肉碱和糖酵解途径的潜在机制。
Esophageal cancer (EC) is a common malignant disease in eastern countries. However, a study of the metabolomic characteristics associated with other biological factors in esophageal squamous cell carcinoma (ESCC) is limited. Interleukin enhancer binding factor 2 (ILF2) and ILF3, double-stranded RNA-binding proteins, have been reported to contribute to the occurrence and development of various types of malignancy. Nevertheless, the underlying functions of ILF2 and ILF3 in ESCC metabolic reprogramming have never been reported. This study aimed to contribute to the metabolic characterization of ESCC and to investigate the metabolomic alterations associated with ILF2 and ILF3 in ESCC tissues. Here, we identified 112 differential metabolites, which were mainly enriched in phosphatidylcholine biosynthesis, fatty acid metabolism, and amino acid metabolism pathways, based on liquid chromatography–mass spectrometry and capillary electrophoresis–mass spectrometry approaches using ESCC tissues and paired para-cancer tissues from twenty-eight ESCC patients. In addition, ILF2 and ILF3 expression were significantly elevated in EC tissues compared to the histologically normal samples, and closely associated with PI3K/AKT and MAPK signaling pathways in ESCC. Moreover, in ESCC tissues with a high ILF2 expression, several short-chain acyl-carnitines (C3:0, C4:0, and C5:0) related to the BCAA metabolic pathway and long-chain acyl-carnitines (C14:0, C16:0, C16:0-OH, and C18:0) involved in the oxidation of fatty acids were obviously upregulated. Additionally, a series of intermediate metabolites involved in the glycolysis pathway, including G6P/F6P, F1,6BP, DHAP, G3P, and 2,3BPG, were remarkably downregulated in highly ILF3-expressed ESCC tissues compared with the corresponding para-cancer tissues. Overall, these findings may provide evidence for the roles of ILF2 and ILF3 during the process of ESCC metabolic alterations, and new insights into the development of early diagnosis and treatment for ESCC. Further investigation is needed to clarify the underlying mechanism of ILF2 and ILF3 on acyl-carnitines and the glycolysis pathway, respectively.
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影响因子：
4.8
作者：
Kiesler, Patricia;Haynes, Paul A.;Vercelli, Donata
通讯作者：
Vercelli, Donata
影响因子：
6.4
作者：
Eliyahu, Galit;Kreizman, Tamar;Degani, Hadassa
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影响因子：
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作者：
Cheng M;Bhujwalla ZM;Glunde K
通讯作者：
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影响因子：
4.3
作者：
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通讯作者：
Ma, Chang-Chun
影响因子：
5.1
作者：
Glunde K;Penet MF;Jiang L;Jacobs MA;Bhujwalla ZM
通讯作者：
Bhujwalla ZM