CSF-1-induced DC-SIGN(+) macrophages are present in the ovarian endometriosis.
CSF-1-induced DC-SIGN(+) macrophages are present in the ovarian endometriosis.
复制标题
CSF-1诱导的DC-SIGN巨噬细胞存在于卵巢子宫内膜异位症中
DOI：
10.1186/s12958-022-00901-w
复制
发表时间：
2022-03-08
期刊：
影响因子：
--
通讯作者：
Min A
中科院分区：
文献类型：
--
作者：
Xiaocui L;Wei H;Yunlang C;Zhenzhen Z;Min A
背景
Background
Researchers have found that macrophages are the predominant cells in the peritoneal fluid (PF) of endometriosis patients. CSF-1 has been found to accumulate in the lesions and PF of endometriosis patients, and CSF-1 induces THP-1-derived macrophages to polarize toward a CD169+ DC-SIGN+ phenotype. Does the cytokine CSF-1 induce monocytes to differentiate into macrophages with a DC-SIGN+ phenotype in endometriosis?
Methods
The level of CSF-1 in the endometrium of control subjects, and the eutopic, and ectopic endometrium of endometriosis patients was evaluated by real-time polymerase chain reaction (qRT–PCR) and was determined by enzyme-linked immunosorbent assay (ELISA) in the PF of control and endometriosis patients. CSF-1 expression was examined with a MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel. DC-SIGN+ macrophages were detected by immunohistochemical staining of tissues and flow cytometric analysis of the PF of control subjects (N = 25) and endometriosis (N = 35) patients. The phenotypes and biological activities of CSF-1 -induced macrophages were compared in an in vitro coculture system with peripheral blood lymphocytes from control subjects.
Results
In this study, we found that the proportion of DC-SIGN+ CD169+ macrophages was higher in the abdominal immune microenvironment of endometriosis patients. CSF-1 was primarily secreted from ectopic lesions and peritoneum in mice with endometriosis. In addition, CSF-1 induced the polarization of macrophages toward a DC-SIGN+ CD169+ phenotype; this effect was abolished by the addition of an anti-CSF-1R antibody. CSF-1 induced the generation of DC-SIGN+ macrophages, leading to a depressed status of peripheral blood lymphocytes, including a high percentage of Treg cells and a low percentage of CD8+ T cells. Similarly, blockade with the anti-CSF-1R antibody abrogated this biological effect.
Conclusions
This is the first study on the role of DC-SIGN+ macrophages in the immune microenvironment of endometriosis. Further study of the mechanism and biological activities of CSF-1-induced DC-SIGN+ macrophages will enhance our understanding of the physiology of endometriosis.
Graphical Abstract
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影响因子：
6.1
作者：
Akoum, Ali;Lawson, C.;Maheux, R.
通讯作者：
Maheux, R.
影响因子：
64.5
作者：
Geijtenbeek, TBH;Torensma, R;Figdor, CG
通讯作者：
Figdor, CG
影响因子：
30.5
作者：
Perdiguero EG;Geissmann F
通讯作者：
Geissmann F
影响因子：
82.9
作者：
通讯作者：
--
DOI：
10.1111/j.1600-0897.2004.00228.x
发表时间：
2004-11-01
影响因子：
3.6
作者：
Mettler, L;Schmutzler, AG;Salmassi, A
通讯作者：
Salmassi, A